I-Recombinant Human TPO Protein
I-ThrombopoietinTpo), eyaziwa nangokuthi i-Megakaryocyte Growth and Development Factor (MGDF), iyi-cytokine elawula ukukhiqizwa kwe-megakaryocyte kanye nokukhiqizwa kweplatelet. I-TPO isebenza kusenesikhathi ekuzibophezeleni kohlu lwe-megakaryocyte, ikhuthaza ukwanda kwamaseli e-megakaryocyte progenitor nokwandisa i-megakaryocyte ploidy. Kodwa-ke, i-TPO ayithinti ukukhululwa kweplatelet kuma-megakaryocyte avuthiwe [1].
I-TPO idlala indima ebalulekile ku-hematopoieticI-Stem Cell(HSC) umthethonqubo (Umfanekiso 1). Ucwaningo luveza ukuthi ukusayinda kwe-TPO kuma-HSC kugcina ukuthula kwe-stem cell ukuze kugcinwe isikhathi eside noma kuqhubekisele phambili ukunwetshwa kwe-HSC phakathi nemicimbi ebalulekile (isb, ukufakelwa kabusha). Kuzo zombili izimo, i-TPO ilungisa ukuguqulwa komjikelezo weseli. Indlela yokusayina ye-TPO-Mpl inomthelela kumetabolism ye-mitochondrial, futhi lo mthethonqubo we-metabolic ungase ube nomthelela ekuhlukaniseni kohlu lozalo lwe-HSC. Ngaphezu kwalokho, ukusayinda kwe-TPO kukhuthaza ukuzivuselela kwe-HSC, kugqamisa amandla ayo okwelapha okwelapha izifo ze-hematopoietic ezibonakala ngokungasebenzi kahle kwe-stem cell, njenge-aplastic anemia, ngokuthuthukiswa kwezidakamizwa ze-TPO-mimetic.
Kusiko lwe-HSC ex vivo, i-TPO ithuthukisa ukwanda kwamaseli. Nakuba i-TPO iyodwa ithinta kancane ukwanda kwezinhlelo zesiko leseli elilodwa, inhlanganisela yayo ne-SCF, IL-3, kanye ne-IL-6 kukhulisa kakhulu ukukhula kwe-HSC clonal (Ithebula 1). Intuthuko yakamuva ithuthukise ukwakheka kwe-cytokine cocktail okuhlanganisa i-TPO yemiphumela ethuthukisiwe yesiko le-HSC [4,5].
Ithebula 1. Imithelela ye-TPO ekwakhekeni kwe-HSC colony in vitro [3].
Izici Zomkhiqizo
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Ukuhlanzeka okuphezulu
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I-bioactivity engavamile
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I-endotoxin ephansi
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Inyumba, i-mycoplasma-free
Ukuqinisekiswa Kokusebenza Komkhiqizo
Umfanekiso 2. Ukuhlaziywa kwe-SDS-PAGE ngaphansi kwezimo zokunciphisa kuqinisekisa > ubumsulwa obungama-90%.
Umfanekiso 3. Ukuhlolwa kokusabalala kwamaseli e-Mo7e: Umkhiqizo we-T&L ubonisa > umsebenzi ongu-5x uma uqhathaniswa nezimbangi ezivela kwamanye amazwe.
Ulwazi Lomkhiqizo
Omqondofana: thrombopoietin, C-mpl ligand, Megakaryocyte colony-stimulating factor (MKCSF), MGDF,THCYT1, MPLLG
Ingxenye #:P40225
Ukulandelana kwe-amino acid: S22-L195 (P157N & R164N) Ithegi: Umaki wakhe ku-C-terminus
Isistimu yokuvezwa: amaseli angu-HEK293 Isisindo semolekyuli: 19.5kDa
Ubumsulwa: > 90% njengoba kunqunywa i-SDS-PAGE
I-Endotoxin: Umsebenzi: Kukalwa ekuhlolweni kokwanda kwamaseli kusetshenziswa amaseli e-MO7e e-megakaryocytic leukemic yabantu, ehambisana nomsebenzi othize we-≥ 5.0×106 IU/mg.
Ukwakhiwa:I-Lyophilized isuka ku-PBS oyinyumba (pH 7.4), ngokuvamile yengezwe ngo-6% - 8% mannitol njengesivikelo.
Ukuzinza Nesitoreji
Impushana ye-Lyophilized: Izinzile kuze kube yizinyanga eziyisi-6 uma igcinwe ku--20°C ngaphansi kwezimo eziyinyumba ngemva kokubuyiselwa kabusha, noma izinyanga eziyi-12 ku--80°C.
Ukubuyisela kabusha: Chaza emanzini angenalutho umjovo, usawoti ovamile, noma i-PBS. Gcina ukuhlushwa okuhlanjululwe ≥ 100 µg/mL.
Ukuphatha: I-Aliquot ukuze ugweme imijikelezo yokuqhwaza ephindaphindiwe.
Sihlosile
Okwezinjongo zocwaningo nezokukhiqiza kuphela.
Izithenjwa
[1] de Graaf CA, Metcalf D. Thrombopoietin kanye ne-hematopoietic stem cell. Umjikelezo Weseli. 2011;10(10):1582-9. doi:10.4161/cc.10.10.15619.
[2] I-Nakamura-Ishizu A, i-Suda T. Izindima eziningi ze-thrombopoietin ku-hematopoietic stem cell regulation. U-Ann NY Acad Sci. 2020;1466(1):51-58. doi:10.1111/nyas.14169.
[3] USitnicka E, et al. Umphumela we-thrombopoietin kumaseli we-murine hematopoietic stem. Igazi. 1996;87:4998-5005. PMID:8652812.
[4] UPineault N, et al. Imiphumela ye-Cytokine ekwandeni kwe-CD34+ yentambo yegazi. I-Cytotherapy. 2010;12:467-80.
[5] Ohmizono Y, et al. I-TPO isebenzisana ne-SCF/flt3 ligand egazini lentambo ukunwetshwa kwe-HSC. I-leukemia. 1997;11:524-30. doi:10.1038/sj.leu.2400588.