I-Recombinant Human TGF-β3 Amaprotheni

Isitoko: Sikhona
Imodeli: GMP-TL646

Xhumana nathi

Incazelo

Izibalo Zedatha

Ukwenziwa kwezinhlamvu

Amafayela Amadokhumenti

Uhlolojikelele Lomkhiqizo

I-Recombinant Human Transforming Growth Factor Beta 3 (TGF-β3) iyi-cytokine esebenzayo ehilelekile ekulawuleni ukukhula kwamangqamuzana, ukuhlukaniswa, kanye nezimpendulo zokuzivikela komzimba. Ngokubophezela kuma-receptors ayo, i-TGF-β3 yenza kusebenze izindlela zokusayina ezansi nomfula ezilawula ukukhiqizwa kwe-matrix engaphandle kwezicubu nokulungisa izicubu, okuyenza ibe ithuluzi elibalulekile ocwaningweni lwebhayoloji yamaselula.

Izici Eziyinhloko

1.Ukuhlanzeka Okuphezulu: Lo mkhiqizo ungaphansi kwezinqubo zokuhlanza eziqinile, ufinyelela izinga lokuhlanzeka elingaphezu kuka-95%, uqinisekisa imiphumela yokuhlola ethembekile nephinde ikhiqizeke.

2.Umsebenzi Ophakeme: Ukuhlolwa kwemisebenzi eminingi kuqinisekisa ukuthi lo mkhiqizo ungenisa ngempumelelo izimpendulo ezilindelekile kumaseli okuqondiwe, afanele izifundo ezihlukahlukene zebhayoloji yamaseli.

I-3.Batch Consistency: Ukukhiqiza okuqinile nezinqubo zokulawula ikhwalithi ziqinisekisa ukuvumelana phakathi kwamaqoqo, kunciphisa ukuhlukahluka kokuhlola.

4.Akunasilwane: Lo mkhiqizo wakhiwe ngaphandle kokusetshenziswa kwanoma yiziphi izingxenye ezithathwe esilwaneni, uhlangabezana nezidingo zamahhala zezilwane futhi ufanele uhla olubanzi lwezicelo zocwaningo.

Izinhlelo zokusebenza

• I-Cell Biology: Isetshenziselwa ukutadisha izinqubo zebhayoloji eziyisisekelo ezifana nokukhula kwamaseli, ukuhlukaniswa, kanye ne-apoptosis.

• Ubunjiniyela Bezicubu: Bukhuthaza ukwakheka kwe-matrix engaphandle kwamangqamuzana nokuvuselelwa kwezicubu, okubalulekile ekulungiseni izicubu kanye nemithi evuselelayo.

• I-Immunology: Iphenya indima ye-TGF-β3 ekulawuleni amasosha omzimba, ihlola ukuthi kungenzeka yini ukuthi isetshenziswe ekwelapheni izifo zokuzivikela komzimba.

• I-Oncology: Icwaninga indima ye-TGF-β3 ku-tumor microenvironment, ihlinzeka ngemininingwane emisha namasu okwelapha umdlavuza.

Imininingwane kanye nokupakishwa

• Ukucaciswa: Kutholakala ngosayizi abahlukahlukene ukuze kuhlangatshezwane nezidingo zokuhlola ezihlukene, ezihlanganisa u-10μg, 50μg, 100μg, kanye no-1mg.

• Ukupakishwa: Kuhlinzekwa ngefomu eliyinyumba eliyi-lyophilized ukuze kube lula ukuyibeka nokuthutha.

Isitoreji Nokuzinza

• Isitoreji: Gcina ku- -20°C noma ngaphansi, ugwema imijikelezo yokuqhwaza ephindaphindiwe.

• Ukuzinza: Umkhiqizo uzinzile ngaphansi kwezimo zokulondoloza ezinconyiwe, okuqinisekisa ukusebenziseka kwesikhathi eside.

Imiyalo Yokusetshenziswa

1.Ngaphambi kokusetshenziswa, ncibilikisa impushana e-lyophilized endaweni efanele oyinyumba endaweni engenalutho.

2.Nciphisa futhi ulungiselele ngokwezidingo zokuhlola.

3.Ukuze uthole imiphumela enembile, ungaxubani neminye imikhiqizo efanayo.

Izixwayiso

• Lo mkhiqizo usetshenziselwa ucwaningo kuphela futhi awuhloselwe ukuxilonga noma ukwelashwa.

• Landela imiyalelo ngokucophelela ukuze ugweme ukuthikameza umsebenzi womkhiqizo.

• Uma uhlangabezana nezinkinga ngesikhathi sokusebenzisa, sicela uxhumane nethimba lethu losekelo lobuchwepheshe ngokushesha.

Ama-Cytokines
Expression Host	HEK293 amaseli
Ukulandelana Kwamaprotheni	Ukulandelana kwe-DNA okubhala ngekhodi i-TGF-β3 yomuntu (P10600 A301-S412) kuvezwe nge-Fc-tag ku-C-terminus.
I-Molecular Mass	Iphrotheni yomuntu ye-TGF-β3 ephinde yahlanganiswa iqukethe ama-amino acid angu-375 futhi ibikezela isisindo samangqamuzana angu-42.1 kD.
QC Testing Ubumsulwa	> 90 % njengoba kunqunywa i-SDS-PAGE
I-Endotoxin
Umsebenzi	Kunqunywa ukukhuthazwa okuncike kumthamo wokwanda okuvimbela i-IL-4 - amaseli egundane e-HT-2 ancike. I-ED₅₀ elindelwe yalo mphumela ngu-≤ 0.1 ng/mL.
Ukwakhiwa	I-Lyophilized kusuka ku-PBS oyinyumba, i-pH 7.4. Ngokuvamile i-6 % i-mannitol yengezwa njengezivikeli ngaphambi kwe-lyophilization.
Ukuzinza	Izinyanga ezingama-24 ku-2℃ kuye ku-8℃ esimweni se-lyophilized. Izinyanga eziyisi-6 ku -20 ℃ ngaphansi kwezimo eziyinyumba ngemva kokwakhiwa kabusha. Izinyanga eziyi-12 ku -80 ℃ ngaphansi kwezimo eziyinyumba ngemva kokwakhiwa kabusha.
Isitoreji	Ncoma ukuthi i-aliquot iphrotheni ibe ngamanani amancane ngemva kokuhlanganiswa kabusha namanzi omjovo, usawoti ovamile noma i-PBS, futhi ugcine ukugxila okuhlanjululwe ngaphezu kuka-100μg/mL. Gwema imijikelezo yokuqhwaza ephindaphindiwe.