I-Recombinant Human IL-2 protein

Isitoko: Sikhona
Imodeli: GMP-TL906

Xhumana nathi Chofoza ukuze uthole Imininingwane Yomkhiqizo

Incazelo

Izibalo Zedatha

Ukwenziwa kwezinhlamvu

Amafayela Amadokhumenti

Amaseli e-T acushiwe ngokuvamile awakwazi ukuphila isikhathi eside kusiko le-in vitro, futhi ukungezwa kwe-IL-2 kungakhuthaza ukwanda kwawo kwesikhathi eside nokuqhubekayo. Ingaphezulu lamaseli e-T amile alivezi i-IL-2R futhi ayiphenduli ku-IL-2; Amaseli e-T acushwe yi-mitogen noma ezinye izisusa zingaveza i-IL-2R futhi abe amaseli okuhlosiwe we-IL-2; I-IL-2 ingaphinda ifake isisho esinyukile se-IL-2R kumaseli okuqondiwe. Isisho se-IL-2R kumaseli e-T singokwesikhashana, ngokuvamile sifinyelela umvuthwandaba ezinsukwini ezingu-2-3 ngemva kokuqaliswa futhi zinyamalale cishe ezinsukwini ezingu-6-10. Ngokunyamalala kwe-IL-2R, ama-T cell alahlekelwa amandla awo okuphendula i-IL-2. Ngakho-ke, ukuze kugcinwe ukukhula kwesikhathi eside kwamaseli e-T avamile ku-vitro, kuyadingeka ukuthi uhlale uvuselela amaseli e-T nge-mitogen noma ezinye izikhuthazi ukuze kugcinwe ukubonakaliswa kwe-IL-2R. Amaseli e-NK yiwona kuphela amaseli e-lymphoid avame ukuveza i-IL-2R, ngakho-ke ahlala egcina ukusebenza kabusha ku-IL-2. Nokho, i-IL-2R kuphela evezwa kumaseli e-NK amile β Chain futhi γ Amaketango anobudlelwane obuphansi be-IL-2 futhi angasabela kuphela ngokugxila okuphezulu kwe-IL-2. Uma amaseli e-NK ecushiwe, aveza i-IL-2R α Chain, abe isamukeli sokuhlangana okuphezulu.

Ama-Cytokines
Expression Host	E.coli
Igama elifanayo	I-TCGF, i-lymphokine, i-Interleukin 2
I-Molecular Mass	Iphrotheni yomuntu ye-IL-2 ephinde yahlanganiswa ibikezela isisindo samangqamuzana esingu-15.4 kD.
QC Testing Ubumsulwa	> 90 % njengoba kunqunywa i-SDS-PAGE.
I-Endotoxin
Umsebenzi	Kunqunywa ukukhuthazwa okuncike kumthamo wokusabalala kwamaseli e-CTLL-2, okuhambisana nomsebenzi we-≥1x10⁷U/mg.
Ukwakhiwa	I-Lyophilized kusuka ku-PBS oyinyumba, i-pH 7.4. Ngokuvamile i-6 % i-mannitol yengezwa njengezivikeli ngaphambi kwe-lyophilization.
Ukuzinza	Ukulungiswa kwe-Lyophilized kungagcinwa ku-2-8 ℃. Izinyanga eziyisi-6 ku -20 ℃ ngaphansi kwezimo eziyinyumba ngemva kokwakhiwa kabusha. Izinyanga eziyi-12 ku -80 ℃ ngaphansi kwezimo eziyinyumba ngemva kokwakhiwa kabusha.
Isitoreji	Ncoma ukuthi i-aliquot iphrotheni ibe ngamanani amancane ngemva kokuhlanganiswa kabusha namanzi omjovo, usawoti ovamile noma i-PBS, futhi ugcine ukugxila okuhlanjululwe ngaphezu kuka-100μg/mL. Gwema imijikelezo yokuqhwaza ephindaphindiwe.