Umpetha Wokulungisa Ezindaweni Zokushisa Ezisezingeni Eliphezulu! I-T&L Biotechnology Yethula Ngokusemthethweni I-New-Generation Thermostable bFGF Protein
I-Basic Fibroblast Growth Factor (bFGF), ilungu lomndeni we-fibroblast growth factor, icupha izindlela zokudlulisa izimpawu ngokubopha kanye nokwenza kusebenze ama-receptors aqondiwe.Iseli T izindawo ezingaphezulu. I-bFGF ilawula ukwanda kwezinhlobo zamaseli ahlukahlukene, okuhlanganisa amaseli endothelial, ama-neurons, ama-chondrocyte, amaseli emisipha abushelelezi, ama-melanocyte, nama-adipocyte. Ukwengeza, i-bFGF ibandakanyeka ezinqubweni zebhayoloji ezifana nokuzivuselela kwamangqamuzana, ukuhlukaniswa, ukuthuthukiswa, ukulungiswa kwezicubu, kanye nokuvuselelwa kabusha. Njengesici esibalulekile sokukhula kwamaseli, i-bFGF isetshenziswa kabanzi kusiko le-in vitro lezinhlobo zamaseli ahlukahlukene.
Ucwaningo lubonisa ukuthi i-bFGF ayizinzile ngaphansi kwezimo zomzimba, nengxenye yempilo cishe yamahora angu-8 kumasiko amaseli ezincelisayo. Ukuzinza okushisayo okuphansi kanye nengxenye yempilo emfushane ye-bFGF kuholela ezinseleleni zesiko lamaseli, ezidinga ukwengezwa kwesici sokukhula njalo noma ukushintshwa okuphakathi kwansuku zonke. Iphrotheni ye-bFGF eshisayo, eklanywe ngokuguqulwa kwe-bFGF yemvelo, ibhekana nale nselele. Igcina umsebenzi onamandla webhayoloji we-bFGF yemvelo ngenkathi ifinyelela ukuqina okushisayo okuthuthukisiwe, igcina ubuqotho besakhiwo nomsebenzi wamaprotheni ebangeni elibanzi lokushisa.
I-embryonic yomuntu I-Stem Cells (hESCs) kanye namaseli esiqu e-pluripotent (iPSC) anamandla okuhlukanisa kuzo zonke izinhlobo zamaseli e-somatic. Ngesikhathi se-in vitro isiko lama-hESC nama-iPSC, i-bFGF ibalulekile ekugcineni i-stem cell pluripotency. Ucwaningo lubonisa ukuthi ama-hESC akhuliswe nge-bFGF e-thermostable enza amakoloni angahlukanisiwe ngokushesha (Fig. 1A) futhi abonise amazinga okukhula athuthukisiwe phakathi nesiko lesikhathi eside (Fig. 1B). Ucwaningo olwengeziwe lwembula ukuthi i-bFGF eshisayo ikhuthaza ukwanda kwe-hESC ngenkathi isekela ukuvezwa komaka be-pluripotency (isb, OCT4, SOX2, KLF4), iqinisekisa ikhono layo lokulondoloza i-hESC pluripotency-isici esibalulekile sezinhlelo zokusebenza zesiko lamaseli.
A: Isimo sokukhula sama-hESC ngoSuku 2 ngaphansi kwezimo zesiko esingaphakeli ngokuhlukahluka okuhlukile kwe-bFGF.
B: Ukuqhathaniswa kwamazinga okukhula kwamaseli phakathi nesiko eliqhubekayo ne-WT-bFGF (uhlobo lwasendle) noma i-TS-bFGF (i-thermostable) ngaphansi kwezimo zokungaphakeli.
Ukwethulwa Komkhiqizo
I-Recombinant Human bFGF Protein (Heat Stable)
Izici Zomkhiqizo
- I-thermostable
- Ukuhlanzeka okuphezulu (>90%)
- Umsebenzi ophezulu
- I-Endotoxin Ephansi (
- Oyinyumba
- I-Mycoplasma-Free
Ukusebenza Komkhiqizo
isicelo Scenario
Uma yengezwe ku-iPSC Culture medium, ukushintshwa okumaphakathi kungenziwa njalo ezinsukwini ezi-2.
Njengoba kuboniswe ku-Fig. 5, i-T&L's Recombinant Human bFGF Protein (GMP-TL901) kanye ne-Thermostable bFGF isetshenziswe ku-AMMS® Pluripotent Stem Cell Medium (AS-35) yesiko le-iPSC. Ngokwesilinganiso esimaphakathi okungezwe nge-bFGF eshisayo, izinguquko zansuku zonke noma zonke ezinye izinsuku ze-coloni zaba ngcono kakhulu izinombolo namasayizi wekholoni uma kuqhathaniswa ne-bFGF evamile. Ngokuphawulekayo, ukusebenza kwamaseli akuzange kubonise umehluko omkhulu phakathi kwezivumelwano zansuku zonke nezansuku zonke ezine-bFGF eshisayo, eqinisekisa ukufaneleka kwayo kwesiko le-iPSC eliyongayo.
Unentshisekelo yokuzama i-New-Generation Thermostable bFGF Protein yethu?
Xhumana nathi namuhla ukuze ucele isampula yamahhala futhi uzizwele izinzuzo mathupha ngocwaningo lwakho!
I-T&L Biotechnology Co., Ltd. igxile ocwaningweni nasekuthuthukisweni kwezisetshenziswa ezingahluziwe zebanga le-GMP kanye nama-reagents amaseli kanye I-Gene Therapy (CGT). Sizibophezele ekunikezeni izixazululo eziphelele zamakhasimende e-CGT ngomkhiqizo wethu ophelele kanye neminikelo yesevisi. Imikhiqizo yethu ihlanganisa ukuhlukaniswa kweseli futhi kusebenze ubuhlalu kazibuthe, amaprotheni e-eukaryotic/prokaryotic recombinant, i-serum-free medium, ama-cell culture kits, futhi nokuningi.
Sisebenzisa ilabhorethri yocwaningo nentuthuko kanye neshabhu yokusebenzela ehlanzekile yeleveli ye-GMP engu-3,200-square. Lezi zakhiwo zihlanganisa izinkundla zokuhlukaniswa kwamaseli ukuthuthukiswa kobuhlalu obuzibuthe, ubunjiniyela be-eukaryotic kanye ne-prokaryotic protein expression, kanye nokuthuthukiswa kwe-serum-free medium. Sinamathela kukho kokubili i-ISO 13485 kanye ne-ISO 9001 Quality Management System Standards. Ukwengeza, eminye yemikhiqizo yethu inefayela le-FDA DMF.