Ingqikithi Yamandla Eseli: 10⁷ ukuya ku-2×10⁹ amaseli.
Amandla Amaseli Anelebuli: 10⁵ kuya ku-10⁸ amaseli (Lapho uhlukanisa amaseli amakhulu kunama-lymphocyte, umthamo wekholomu yokuhlukanisa ungase wehle).
Ivolumu EngenaluthoUmthamo: 1.35 ml.
Ivolumu ye-Reservoirawu :7ml.
Isilinganiso sokugeleza: Ku-1x PBS equkethe u-0.5% BSA (Bovine Serum Albumin): 1.2 - 2.1 ml/min.
Ibanga Elisebenzayo: Isebenza kumasampuli anobubanzi obungaphansi kuka-30 μm.
Ubunyumba: Amakholomu okuhlukanisa ahlanganiswe ngawodwana ngendlela eyinyumba.
Amakholomu Okuhlukaniswa kwe-MaxSortin® L asetshenziswa ngokuhlanganiswa nochungechunge lwe-MaxSortin® lwamaseli okuhlunga ubuhlalu kazibuthe namabhafa okuhlunga amaseli. I-MaxSortin® L-Separation Columns ingakwazi ukuhlunga ngobumnene amaseli aboshwe ubuhlalu obukazibuthe.
Izinqubo Zokuhlola:
1. Thatha Ikholomu Yokuhlukaniswa kwe-MaxSortin® L (MS-CL01) futhi uyibeke kusihlungi se-MACS (130-090-976), bese uyihlanza kabili nge-1 mL ye-MaxSortin® cell sorter buffer.
2. Engeza isampula, efakwe ngochungechunge lwe-MaxSortin® lwamaseli okuhlunga ubuhlalu kazibuthe namaseli, kuKholamu Yokuhlukaniswa kuka-L. Ngemva kokugeleza ngokwemvelo, engeza isigcinalwazi sokuhlela iseli zibe izingxenye ezimbili, 3 mL isikhathi ngasinye. Qoqa ukungcola ngeshubhu engu-15 ml. Amaseli aqoqiwe amaseli akhethwe kabi.
3. Ngemva kokuthi isilondolozi sokuhlunga seseli sesiphumile, khipha Ikholomu Yokuhlukanisa L kusihlungi se-MACS bese usifaka kwelinye ishubhu elisha le-centrifuge elingu-15 mL (liphawule kahle). Engeza u-3 mL webhafa yokuhlunga amaseli Kukholomu Yokuhlukanisa L futhi usebenzise i-plunger ukuze uphushe uketshezi luphume ngokuqondile. Amaseli aqoqiwe amaseli akhethwe kahle.
4. Bala amaseli futhi uqhube ukutholwa kwe-cytometry yokugeleza.
Lo mkhiqizo usebenza kuphela ku-in vitro cell culture futhi awukwazi ukusetshenziselwa ngokuqondile ukwelashwa komtholampilo.
