| Igama | Inombolo ye-athikili | Ukucaciswa | Izimo Zokugcina | I-Shelf Life |
| Ubuhlalu bukaMagnetic bokuhlukaniswa kwe-MaxSortin® CD4 | I-TL-623 | 2 mL ngesamba samaseli angu-1×10⁹ | 2 - 8 °C | 6 izinyanga |
| I-MaxSortin® Cell Sorting Buffer | I-MS-BF100 | 100 ml | 2 - 8 °C | Izinyanga ezingu-12 |
| Ikholomu Yokuhlukanisa ye-MaxSortin® L | I-MS-CL01 | 1 isiqephu | 10 - 35 °C | Izinyanga ezingu-12 |
Izinhlobo Ezisebenzayo: Umuntu
I-Magnetic Bead Endotoxin:
Ukubukeka kwe-Magnetic Bead: Uketshezi olunsundu
I-MaxSortin® CD4 Separation Kit ingasetshenziswa ukuhlunga amaseli e-CD4+ T omuntu. Ngokufukamela ubuhlalu obuzibuthe obuhlukanisa i-nanoscale CD4 ngamaseli, ukuhlungwa kwamaseli e-CD4+ T kungafinyelelwa. Ubuhlalu obuzibuthe bokuhlukaniswa kwe-nanoscale CD4 bufukanyelwa ngamaseli egazi we-peripheral mononuclear cell (PBMCs) bese buhlukaniswa nozibuthe, okwenza kube nokuhlukaniswa nokunothisa kwamaseli e-CD4+ T, okudlala indima ekuhlanzeni amaseli e-CD4+ T futhi kuyasebenza ekukhiqizeni imikhiqizo yokwelapha ngamaseli.
1. Misa kabusha ama-PBMC abantu kubhafa ye-PBS equkethe u-1% we-albumin ye-human serum (HSA). Thatha isampula yokubala bese udlulisela amaseli angu-1×10⁷ kushubhu ye-EP engu-1.5 mL. Bese i-centrifuge ku-1500 rpm imizuzu emi-5.
2. Lahla amandla angaphezu kwavamile, thatha u-80 μL we-MaxSortin® Cell Sorting Buffer ukuze umise kabusha amaseli, engeza u-20 μL wobuhlalu obuzibuthe bokuhlukaniswa kwe-CD4, uhlanganise kahle bese ububeka esiqandisini ngo-2 - 8 °C ukuze kufakwe incubation imizuzu engu-15.
3. Thatha Ikholomu Yokuhlukanisa I-MaxSortin® L bese uyibeka kusihlungi seseli esisebenza zibuthe (i-MACS), bese uyihlanza kabili nge-1 mL ye-MaxSortin® Cell Sorting Buffer.
4. Thatha isampula esiqede ukufukamela ngaphandle kwefriji ngo-2 - 8 °C, engeza i-1 mL yesisombululo se-MACS, i-centrifuge ku-1500 rpm imizuzu engu-5, futhi ulahle amandla amakhulu.
5. Engeza u-1 mL we-MaxSortin® Cell Sorting Buffer ukuze umise kabusha amaseli, engeza isampula kukholamu yokuhlukanisa. Ngemva kokuba igeleze ngokwemvelo, engeza i-MaxSortin® Cell Sorting Buffer ngezingxenye ezimbili, 1 mL isikhathi ngasinye. Qoqa ukungcola ngeshubhu le-centrifuge elingu-15 mL.
6. Ngemva kokuthi i-MaxSortin® Cell Sorting Buffer isiphumile, khipha ikholomu ehlukanisayo kusihlungi se-MACS bese usifaka kwelinye ishubhu elisha le-centrifuge elingu-15 mL. Engeza u-3 mL we-MaxSortin® Cell Sorting Buffer kukholamu yokuhlukanisa futhi usebenzise ipiston eza nekholomu yokuhlukanisa ukuze uphushe uketshezi luphume ngokuqondile.
7. Beka ishubhu le-centrifuge elingu-15 mL eliqukethe uketshezi oluqoqiwe ku-centrifuge evundlile kanye ne-centrifuge ku-1500 rpm imizuzu emi-5.
8. Ngemva kwe-centrifugation, thela i-supernatant, thatha i-1 mL ye-1× ye-Dulbecco's phosphate-buffered saline (DPBS) isisombululo sokumisa kabusha amaseli, ubale amaseli futhi uqhube ukutholwa kwe-cytometry yokugeleza.
Lapho ufukamela ubuhlalu kazibuthe ngamaseli, kuyadingeka ukuxutshwa kahle ukuze kuthuthukiswe ukusebenza kahle kokuhlunga.
