| Igama | Inombolo ye-athikili | Ukucaciswa | Izimo Zokugcina | I-Shelf Life |
| I-MaxSortin® CD8 Separation Magnetic Ubuhlalu | I-TL-624 | 2 mL ngesamba samaseli angu-1×10⁹ | 2 - 8 °C | 6 izinyanga |
| I-MaxSortin® Cell Sorting Buffer | I-MS-BF100 | 100 ml | 2 - 8 °C | Izinyanga ezingu-12 |
| Ikholomu Yokuhlukanisa ye-MaxSortin® L | I-MS-CL01 | 1 isiqephu | 10 - 35 °C | Izinyanga ezingu-12 |
Izinhlobo Ezisebenzayo: Umuntu
I-Magnetic Bead Endotoxin:
Ukubukeka kwe-Magnetic Bead: Uketshezi olunsundu
I-MaxSortin® CD8 Separation Kit ingasetshenziswa ukuhlunga amaseli e-CD8+ T omuntu. Ngokufaka ubuhlalu obuzibuthe obuhlukanisa i-nanoscale CD8 ngamaseli, ukuhlungwa kwamaseli e-CD8+ T kungafinyelelwa. Ubuhlalu obuzibuthe bokuhlukaniswa kwe-nanoscale CD8 bufukanyelwa ngamaseli egazi we-peripheral mononuclear cell (PBMCs) bese bubhekana nokuhlukaniswa kazibuthe, okuvumela ukuhlukaniswa nokunothisa kwamaseli e-CD8+ T, okudlala indima ekuhlanzeni amaseli e-CD8+ T futhi kuyasebenza ekukhiqizweni kwemikhiqizo yokwelapha ngamaseli.
1. Misa kabusha ama-PBMC abantu kubhafa ye-PBS equkethe u-1% we-albumin ye-human serum (HSA). Thatha isampula yokubala bese udlulisela amaseli angu-1×10⁷ kushubhu ye-EP engu-1.5 mL. Bese i-centrifuge ku-1500 rpm imizuzu emi-5.
2. Lahla amandla angaphezu kwavamile, thatha u-80 μL we-MaxSortin® Cell Sorting Buffer ukuze umise kabusha amaseli, engeza u-20 μL wobuhlalu obuyizibuthe bokuhlukaniswa kwe-CD8, uhlanganise kahle bese ububeka esiqandisini ngo-2 - 8 °C ukuze kufakwe incubation imizuzu engu-15.
3. Thatha Ikholomu Yokuhlukanisa I-MaxSortin® L bese uyibeka kusihlungi seseli esisebenza zibuthe (i-MACS), bese uyihlanza kabili nge-1 mL ye-MaxSortin® Cell Sorting Buffer.
4. Thatha isampula eqede ukufukamela uyikhiphe esiqandisini ngo-2 - 8 °C, engeza u-1 mL we-MaxSortin® Cell Sorting Buffer, i-centrifuge ku-1500 rpm imizuzu emi-5, bese ulahla amandla amakhulu.
5. Engeza u-1 mL we-MaxSortin® Cell Sorting Buffer ukuze umise kabusha amaseli, engeza isampula kukholamu yokuhlukanisa. Ngemva kokuba igeleze ngokwemvelo, engeza i-MaxSortin® Cell Sorting Buffer ngezingxenye ezimbili, 1 mL isikhathi ngasinye. Qoqa ukungcola ngeshubhu le-centrifuge elingu-15 mL.
6. Ngemva kokuthi i-MaxSortin® Cell Sorting Buffer isiphumile, khipha ikholomu ehlukanisayo kusihlungi se-MACS bese usifaka kwelinye ishubhu elisha le-centrifuge elingu-15 mL. Engeza u-3 mL we-MaxSortin® Cell Sorting Buffer kukholamu yokuhlukanisa futhi usebenzise ipiston eza nekholomu yokuhlukanisa ukuze uphushe uketshezi luphume ngokuqondile.
7. Beka ishubhu le-centrifuge elingu-15 mL eliqukethe uketshezi oluqoqiwe ku-centrifuge evundlile kanye ne-centrifuge ku-1500 rpm imizuzu emi-5.
8. Ngemva kwe-centrifugation, thela i-supernatant, thatha i-1 mL ye-1× ye-Dulbecco's phosphate-buffered saline (DPBS) isisombululo sokumisa kabusha amaseli, ubale amaseli futhi uqhube ukutholwa kwe-cytometry yokugeleza.
1. Uma ufukamela ubuhlalu kazibuthe ngamaseli, kuyadingeka ukuxutshwa kahle ukuze kuthuthukiswe ukusebenza kahle kokuhlunga.
2. Ikholomu yebhafa neyokwehlukanisa efakwe kule khithi ingasetshenziselwa isilingo sokuqala. Uma imisebenzi yokuhlola eyengeziwe ihilelekile, sicela uthenge i-MaxSortin® Cell Sorting Buffer (Inombolo Yesihloko: MS-BF100) kanye Nekholomu Yokuhlukaniswa Kwe-MaxSortin® (Inombolo Yesihloko: MS-CL01) ngokuhlukana.
Lo mkhiqizo usebenza kuphela ku-in vitro cell culture futhi awukwazi ukusetshenziselwa ngokuqondile ukwelashwa komtholampilo.
