| Igama | Inombolo ye-athikili | Ukucaciswa | Izimo Zokugcina | I-Shelf Life |
| I-MaxSortin® CD138 Separation Magnetic Beads | TL-811-1000 | 1 mL ngesamba samaseli angu-1×10⁹ | 2 - 8 °C | 6 izinyanga |
| I-MaxSortin® Cell Sorting Buffer | I-MS-BF100 | 100 ml | 2 - 8 °C | Izinyanga ezingu-12 |
| Ikholomu Yokuhlukanisa ye-MaxSortin® L | I-MS-CL01 | 1 isiqephu | 10 - 35 °C | Izinyanga ezingu-12 |
Izinhlobo Ezisebenzayo: Umuntu
I-Magnetic Bead Endotoxin:
Ukubukeka kwe-Magnetic Bead: Uketshezi olunsundu
I-MaxSortin® CD138 Separation Magnetic Bead ingasetshenziselwa ukuhlunga amaseli e-CD138+. Ngokuhlanganisa amasosha omzimba angama-CD138 angama-monoclonal ebuhlalu kazibuthe, ewafukamela ngamaseli, bese enza ukuhlukanisa kazibuthe, ukuhlungwa kwamaseli e-CD138+ kungafinyelelwa.
1. Misa kabusha isampula yeseli elixubile lama-PBMC aqukethe cishe u-10% U266 kubhafa ye-PBS equkethe u-1% we-albumin yabantu (HSA). Thatha isampula ukuze ubale bese udlulisela amaseli angu-1×10⁷ kushubhu ye-Ep engu-1.5 mL. Bese i-centrifuge ku-1500 rpm imizuzu emi-5.
2. Lahla amandla angaphezu kwavamile, thatha u-90 μL wesixazululo se-MaxSortin® Cell Sorting Buffer ukuze umise kabusha amaseli, engeza u-10 μL Wokuhlukanisa Ubuhlalu Bezibuthe beMaxSortin® CD138, uhlanganise kahle bese ububeka esiqandisini ngo-2 - 8 °C ukuze kufakwe incubation imizuzu engu-15.
3. Thatha Ikholomu Yokuhlukanisa I-MaxSortin® L bese uyibeka kusihlungi seseli esisebenza zibuthe (i-MACS), bese uyihlanza kabili nge-1 mL ye-MaxSortin® Cell Sorting Buffer.
4. Thatha isampula eqede ukufukamela uyikhiphe esiqandisini ngo-2 - 8 °C, engeza u-1 mL we-MaxSortin® Cell Sorting Buffer, i-centrifuge ku-1500 rpm imizuzu emi-5, bese ulahla amandla amakhulu.
5. Engeza u-1 mL we-MaxSortin® Cell Sorting Buffer ukuze umise kabusha amaseli, engeza isampula Kukholomu Yokuhlukaniswa Kwe-MaxSortin® L. Ngemva kokuba igeleze ngokwemvelo, engeza i-MaxSortin® Cell Sorting Buffer izingxenye ezimbili, 3 mL isikhathi ngasinye, ukuze ugeze amaseli anamathela kukholamu.
6. Ngemva kokuthi i-MaxSortin® Cell Sorting Buffer isiphumile, susa Ikholomu Yokuhlukanisa I-MaxSortin® L kusihlungi se-MACS futhi uyibeke kushubhu ye-centrifuge engu-15 mL (imake kahle). Engeza u-3 mL we-MaxSortin® Cell Sorting Buffer kukholomu Yokuhlukanisa ye-MaxSortin® L futhi usebenzise ipiston ukuze uphushe uketshezi luphume ngokuqondile. Amaseli aqoqiwe amaseli e-CD138+.
7. Bala amaseli futhi uqhube ukutholwa kwe-cytometry yokugeleza.
1. Uma ufukamela ubuhlalu kazibuthe ngamaseli, kuyadingeka ukuxutshwa kahle ukuze kuthuthukiswe ukusebenza kahle kokuhlunga.
2. Uma ingxenye yamaseli e-CD138+ ingaphansi kuka-3%, kutuswa ukuba wengeze u-5 μL wobuhlalu obuzibuthe. Uma ingxenye yamaseli e-CD138+ ingaphezu kuka-3%, kutuswa ukuthi wengeze u-10 μL wobuhlalu kazibuthe.
3.Lo mkhiqizo awukwazi ukusetshenziselwa ukwelashwa komtholampilo.
