I-ActCel T Cell Activation Reagent

I-ActCel T Cell Activation Reagent ihlanganiswa ngokuhlanganisa i-nanatrix ene-anti-body CD3 antibody kanye ne-anti-human CD28, futhi ingenza ama-T cell noma ama-PBMC asebenze ngobumnene nangempumelelo (Peripheral Blood Mononuclear Cells).

Izinqubo Zokuhlola:
1.1 Yenza isilingo ngesihloko esingu-1:200. Amanani athile angeziwe aboniswa kuthebula elilandelayo:

1.2 Ukuthatha u-1×10⁶ T amaseli njengesibonelo: Engeza u-995 μL we-X-VIVOTM 15 medium kanye no-5 μL we-T cell activation reagent (equkethe 200 IU/mL IL-2) kuya ku-1×10⁶ T amaseli. Bese uwasika ku-37 °C ngaphansi kuka-5% CO₂ izinsuku ezi-2.
1.3 Ngemva kokutshala izinsuku ezingu-2 - 3, i-centrifuge ku-300g imizuzu engu-10, khipha amandla amakhulu, bese wengeza u-2 mL we-medium fresh ephelele ukuze ususe i-reagent ye-T cell activation. Bese uqhubeka nokutshala ku-37 °C ngaphansi kuka-5% CO₂.
1.4 Njalo ezinsukwini ezi-2, i-pipette ukumiswa kweseli kahle ukuze uhlanganise ngokulinganayo, engeza izikhathi ezi-2 ivolumu ye-medium entsha ephelele (isilinganiso sevolumu yasekuqaleni kuya kuvolumu yokwengeza ngu-1: 2), futhi ubale amaseli ukubala isici sokukhulisa. Bese uwafake ku-37 °C, 5% CO₂ incubator. Isibonelo, uma i-medium yengezwa ngosuku lwesi-4, futhi ivolumu yokuqala ingu-2 mL, u-4 mL we-medium kufanele yengezwe, futhi ingqikithi yevolumu yokugcina izoba ngu-6 mL.

Lo mkhiqizo usebenza kuphela ku-in vitro cell culture futhi awukwazi ukusetshenziselwa ngokuqondile ukwelashwa komtholampilo.