Intshatsheli yoLungiso kwiNdawo zoBubushushu obuPhezulu! I-T&L Biotechnology Yazisa ngokusesikweni isiZukulwana esitsha se-BFGF Protein
I-Basic Fibroblast Growth Factor (bFGF), ilungu le-fibroblast growth factor family, ibangela umqondiso weendlela zokudluliselwa ngokubophelela kunye nokuvula i-receptors ekujoliswe kuyo.Iseli T imiphezulu. I-bFGF ilawula ukwanda kweentlobo zeeseli ezahlukeneyo, kubandakanywa iiseli ze-endothelial, i-neurons, i-chondrocytes, iiseli ze-muscle ezigudileyo, i-melanocytes, kunye ne-adipocytes. Ukongeza, i-bFGF ibandakanyeka kwiinkqubo zebhayoloji ezinjengokuhlaziya iseli, ukwahlula, uphuhliso, ukulungiswa kwezicubu, kunye nokuvuselelwa. Njengomba obalulekileyo wokukhula kweeseli, i-bFGF isetyenziswa ngokubanzi kwinkcubeko ye-in vitro yeentlobo ezahlukeneyo zeeseli.
Uphononongo lubonisa ukuba i-bFGF ayizinzanga phantsi kweemeko zomzimba, kunye nesiqingatha sobomi obumalunga neeyure ezisi-8 kwiinkcubeko zeeseli ezanyisayo. Uzinzo oluphantsi lwe-thermal kunye nesiqingatha sobomi obufutshane be-bFGF bukhokelela kwimiceli mngeni kwinkcubeko yeeseli, efuna ukongezwa kwemeko yokukhula rhoqo okanye ukutshintshwa okuphakathi kwemihla ngemihla. Iprotheyini ye-bFGF ye-thermostable, eyenziwe ngokuguqulwa kwe-bFGF yendalo, ijongana nalo mngeni. Igcina umsebenzi onamandla webhayoloji ye-bFGF yendalo ngelixa ifezekisa uzinzo olongezelelweyo lobushushu, igcina imfezeko yesakhiwo kunye nomsebenzi weprotheyini kuluhlu olubanzi lobushushu.
I-embryonic yomntu Iiseli zokumas (hESCs) kunye neeseli ze-pluripotent stem (iPSCs) zinamandla okwahlula kuzo zonke iintlobo zeeseli ze-somatic. Ngexesha lenkcubeko ye-in vitro yee-hESC kunye nee-iPSCs, i-bFGF ibalulekile ekugcineni i-stem cell pluripotency. Uphando lubonisa ukuba i-hESCs ekhuliswe kunye ne-bFGF ye-thermostable yenze iikholoni ezingafaniyo ngokukhawuleza (Umfanekiso we-1A) kwaye ubonise amazinga okwandisa okuphuculweyo ngexesha lenkcubeko yexesha elide (Umfanekiso 1B). Uphononongo olongezelelweyo lubonisa ukuba i-bFGF ye-thermostable ikhuthaza ukwanda kwe-hESC ngelixa igcina ukubonakaliswa kweempawu ze-pluripotency (umzekelo, i-OCT4, i-SOX2, i-KLF4), iqinisekisa amandla ayo okugcina i-hESC i-pluripotency-into ebalulekileyo kwizicelo zenkcubeko yeseli.
A: Ubume bokukhula kwee-hESCs ngoSuku lwe-2 phantsi kweemeko zenkcubeko ezixhasayo kunye neentlobo ezahlukeneyo ze-bFGF.
B: Ukuthelekiswa kwamazinga okukhula kweeseli ngexesha lenkcubeko eqhubekayo kunye ne-WT-bFGF (uhlobo lwasendle) okanye i-TS-bFGF (i-thermostable) phantsi kweemeko ze-feeder-free.
Product Launch
I-Recombinant Human bFGF Protein (Ubushushu obuzinzile)
Iimpawu zeMveliso
- IThermostable
- Ukucoceka okuphezulu (>90%)
- Umsebenzi ophezulu
- I-Endotoxin ePhantsi (
- Unyumba
- Mycoplasma-Free
Ukusebenza kweMveliso
pplication Imeko
Xa ifakwe kwi-iPSC yenkcubeko medium, ukutshintshwa okuphakathi kunokwenziwa rhoqo ngeentsuku ezi-2.
Njengoko kuboniswe kwi-Fig 5, i-T & L's Recombinant Human bFGF Protein (GMP-TL901) kunye ne-Thermostable bFGF isetyenziswe kwi-AMMS® Pluripotent Stem Cell Medium (AS-35) yenkcubeko ye-iPSC. Phakathi kuncediswe bFGF thermostable, mihla okanye zonke-olunye-olunye-usuku medium utshintsho kakhulu aphuculwe amanani koloni kunye nobukhulu xa kuthelekiswa bFGF eqhelekileyo. Ngokucacileyo, ukusebenza kweeseli akubonakalisi mahluko ubalulekileyo phakathi kwemihla ngemihla kunye neyolunye usuku lweprothokholi ene-thermostable bFGF, eqinisekisa ukufaneleka kwayo kwinkcubeko ye-iPSC eyonga iindleko.
Ngaba unomdla ekuzameni iProtein yethu ye-Thermostable bFGF yesiZukulwana esitsha?
Qhagamshelana nathi namhlanje ukuze ucele isampuli yasimahla kwaye ube namava eenzuzo ngokwakho kuphando lwakho!
I-T&L Biotechnology Co., Ltd. igxile kuphando kunye nophuhliso lokunyuka komsinga, imathiriyeli ekrwada ye-GMP-grade kunye nezixhobo ezisebenza kwiseli kunye. Unyango lweGene (CGT). Sizimisele ukubonelela ngezisombululo ezipheleleyo kubathengi beCGT ngemveliso yethu ebanzi kunye neenkonzo ezinikezelayo. Iimveliso zethu ziquka ukwahlukana kweeseli kwaye kusebenze amaso kazibuthe, i-eukaryotic/prokaryotic recombinant proteins, i-serum-free medium, izixhobo zenkcubeko yeeseli, kunye nokuninzi.
Sisebenzisa ilabhoratri yophando kunye nophuhliso kunye neworkshop ecocekileyo ye-GMP yenqanaba le-3,200-square-metres. Ezi zixhobo ziquka amaqonga ophuhliso lweeseli zokwahlula amaso obuzibuthe, ubunjineli beeukaryotic kunye neprokaryotic protein, kunye nophuhliso oluphakathi olungenaserum. Sibambelela kuzo zombini i-ISO 13485 kunye ne-ISO 9001 yeMigangatho yeNkqubo yoLawulo loMgangatho. Ukongeza, ezinye zeemveliso zethu zineefayili ze-FDA DMF.