| Igama | Inombolo yenqaku | Inkcazo | Iimeko zokuGcina | Beka ubomi kwishelufa |
| I-MaxSortin® CD4 yoKwahlula amaso eMagnetic | TL-623 | I-2 mL ye-1 × 10⁹ iiseli zizonke | 2 - 8 °C | Iinyanga ezi-6 |
| I-MaxSortin® yoHlulo lweSeli yesithinteli | MS-BF100 | 100 ml | 2 - 8 °C | Iinyanga ezili-12 |
| IKholam yokwahlula iMaxSortin® L | MS-CL01 | Iqhekeza eli-1 | 10 - 35 °C | Iinyanga ezili-12 |
Iintlobo ezisebenzayo: Umntu
I-Magnetic Bead Endotoxin:
Imbonakalo yeMaso yeMagnetic: Ulwelo olumdaka
I-MaxSortin® CD4 Separation Kit inokusetyenziselwa ukuhlela iiseli ze-CD4+ T zabantu. Ngokufukamela i-nanoscale CD4 yokwahlula amaso kazibuthe ngeeseli, ukuhlelwa kweeseli ze-CD4+ T kunokufezekiswa. I-nanoscale CD4 yokwahlula amaso magnetic incubated nge peripheral blood mononuclear cells (PBMCs) kwaye emva koko ixhomekeke ekuhlukaneni kwamagnetic, eyenza ukwahlula kunye nokutyebisa kweeseli ze-CD4 + T, zidlala indima ekuhlambululeni iiseli ze-CD4 + T kwaye zisebenza kwimveliso yeemveliso zonyango lweseli.
1. Ukurhoxisa kwakhona ii-PBMC zabantu kwi-PBS buffer ene-1% ye-albumin ye-human serum (HSA). Thatha isampuli yokubala kwaye udlulisele i-1 × 10⁷ iiseli kwi-tube ye-1.5 mL EP. Emva koko i-centrifuge kwi-1500 rpm imizuzu emi-5.
2. Lahla i-supernatant, thatha i-80 μL ye-MaxSortin® Cell Sorting Buffer ukumisa kwakhona iiseli, yongeza i-20 μL ye-CD4 yokwahlula amaso kazibuthe, xuba kakuhle uze uwabeke kwisikhenkcisi nge-2 - 8 °C ngokufukama imizuzu eli-15.
3. Thatha iKholamu yoKwahlula i-MaxSortin® L uze uyibeke kwisihlungi sokuhlela iiseli esisebenza kumagnetic (MACS), kwaye uyihlakulele kabini nge-1 mL ye-MaxSortin® Cell Sorting Buffer.
4. Thatha isampuli egqityiweyo yokufukamela ngaphandle kwefriji kwi-2 - 8 ° C, yongeza i-1 mL yesisombululo se-MACS, i-centrifuge kwi-1500 rpm imizuzu emi-5, kwaye ulahle i-supernatant.
5. Yongeza i-1 mL ye-MaxSortin® Cell Sorting Buffer ukumisa kwakhona iiseli, yongeza isampuli kwikholamu yokwahlula. Emva kokuba impompoza ngokwemvelo, yongeza i-MaxSortin® Cell Sorting Buffer kwizahlulo ezibini, 1 mL ixesha ngalinye. Qokelela amanzi amdaka kwityhubhu ye-centrifuge eyi-15 ml.
6. Emva kokuba yonke i-MaxSortin® Cell Sorting Buffer iye yaphuma, susa ikholamu yokwahlula kwisihlungi se-MACS kwaye uyibeke kwenye ityhubhu entsha ye-15 mL ye-centrifuge. Yongeza i-3 mL ye-MaxSortin® Cell Sorting Buffer kwikholamu yokwahlula kwaye usebenzise ipiston eza nekholamu yokwahlula ukutyhala ulwelo ngaphandle ngokuthe ngqo.
7. Beka i-tube ye-15 mL ye-centrifuge equlethe i-liquid eqokelelweyo kwi-centrifuge ethe tye kunye ne-centrifuge kwi-1500 rpm imizuzu emi-5.
8. Emva kwe-centrifugation, uthele i-supernatant, thabatha i-1 mL ye-1 × i-Dulbecco ye-phosphate-buffered saline (DPBS) isisombululo sokumisa kwakhona iiseli, ukubala iiseli kunye nokuqhuba ukufunyanwa kwe-cytometry.
Xa ufaka amaso amagnetic ngeeseli, kuyimfuneko ukuxuba ngokucokisekileyo ukuphucula ukusebenza kakuhle kokuhlelwa.
