'Mampoli oa Litokiso Mabakeng a Mocheso o Phahameng! T&L Biotechnology e Tseba Semmuo Protheine ea Moloko o Mocha oa Thermostable bFGF
Basic Fibroblast Growth Factor (bFGF), setho sa lelapa la fibroblast growth factor, e etsa hore ho be le lipontšo tsa phetisetso ka ho tlama le ho kenya tšebetsong li-receptor tse lebelletsoeng.T Seleng bokaholimo. bFGF e laola ho ata ha mefuta e fapaneng ea lisele, ho kenyelletsa lisele tsa endothelial, neurons, chondrocyte, lisele tse boreleli tsa mesifa, melanocyte le adipocyte. Ntle le moo, bFGF e kenya letsoho lits'ebetsong tsa tlhaho tse kang ho inchafatsa lisele, karohano, nts'etsopele, tokiso ea lisele le nchafatso. E le ntlha ea bohlokoa bakeng sa kholo ea lisele, bFGF e sebelisoa haholo moetlong oa in vitro oa mefuta e fapaneng ea lisele.
Boithuto bo bonts'a hore bFGF ha e tsitsa tlasa maemo a 'mele, ka halofo ea bophelo ea lihora tse ka bang 8 litsong tsa lisele tsa mammalian. The tlaase mocheso botsitso le e khutsoanyane halofo ea bophelo ba bFGF lebisa liphephetsong setso sa lisele, e hlokang khafetsa kgolo ntlha tlatsetso kapa letsatsi le letsatsi seaplane phetolo. Protheine ea bFGF e thermostable, e entsoeng ka ho fetola bFGF ea tlhaho, e sebetsana le phephetso ena. E boloka ts'ebetso e matla ea bioloji ea bFGF ea tlhaho ha e ntse e fihlela botsitso bo matlafalitsoeng ba mocheso, ho boloka botsitso ba sebopeho le ts'ebetso ea protheine ho pholletsa le mocheso o pharalletseng.
Embryonic ea motho Stem Cells (hESCs) le induced pluripotent stem cell (iPSCs) li na le monyetla oa ho khetholla mefuta eohle ea lisele tsa somatic. Nakong ea in vitro culture ea hESCs le iPSCs, bFGF e bohlokoa bakeng sa ho boloka stem cell pluripotency. Lipatlisiso li bontša hore li-hESC tse hlahisitsoeng ka thermostable bFGF li theha likolone tse sa arohaneng ka potlako (setšoantšo sa 1A) le ho bonts'a litekanyetso tse ntlafetseng tsa ho ata nakong ea setso sa nako e telele (setšoantšo sa 1B). Liphuputso tse ling li senola hore thermostable bFGF e khothalletsa ho ata ha hESC ha e ntse e boloka pontšo ea matšoao a pluripotency (mohlala, OCT4, SOX2, KLF4), e tiisang bokhoni ba eona ba ho boloka pluripotency ea hESC-e leng karolo ea bohlokoa bakeng sa likopo tsa setso sa lisele.
A: Boemo ba khōlo ea li-hESC ka Letsatsi la 2 tlas'a maemo a setso a se nang lijo le mefuta e fapaneng ea bFGF.
B: Papiso ea litekanyetso tsa kholo ea lisele nakong ea setso se tsoelang pele le WT-bFGF (mofuta oa hlaha) kapa TS-bFGF (thermostable) tlas'a maemo a se nang lijo.
Ho Qala Sehlahisoa
Recombinant Human bFGF Protein (Heat Stable)
Likarolo tsa Sehlahisoa
- Thermostable
- Bohloeki bo Phahameng (>90%)
- Mosebetsi o Phahameng
- Endotoxin e Tlase (
- E nyopa
- Mycoplasma-Mahala
Ts'ebetso ea Sehlahisoa
pplication Scenario
Ha e eketsoa ho iPSC setso medium, phetisetso e mahareng e ka etsoa matsatsi a mang le a mang a 2.
Joalokaha ho bontšitsoe setšoantšong sa 5, T & L's Recombinant Human bFGF Protein (GMP-TL901) le Thermostable bFGF e sebelisitsoe ho AMMS® Pluripotent Stem Cell Medium (AS-35) bakeng sa setso sa iPSC. Boemong bo mahareng, bo tlatselletsoang ka bFGF e thermostable, letsatsi le letsatsi kapa letsatsi le leng le le leng le fetoha ka mokhoa o ntlafetseng haholo oa lipalo le boholo ba likolone ha li bapisoa le bFGF e tloaelehileng. Ka ho hlakileng, ho sebetsa ha lisele ho ne ho sa bonts'a phapang e kholo lipakeng tsa liprothokholo tsa letsatsi le letsatsi le tsa letsatsi le leng le le leng tse nang le thermostable bFGF, e netefatsang ho tšoaneleha ha eona bakeng sa setso se sebetsang hantle sa iPSC.
Na u khahlehela ho leka Protein ea rona ea New-Generation Thermostable bFGF?
Ikopanye le rona kajeno ho kopa sampole ea mahala 'me u bone melemo ea lipatlisiso tsa hau!
T&L Biotechnology Co., Ltd. e tsepamisitse maikutlo ho lipatlisiso le nts'etsopele ea lisebelisoa tse tala tsa sehlopha sa GMP le li-reagents tsa sele le. Gene Therapy (CGT). Re ikemiselitse ho fana ka tharollo e felletseng bakeng sa bareki ba CGT ka lihlahisoa tsa rona tse felletseng le lits'ebeletso. Lihlahisoa tsa rona li kenyelletsa karohano ea lisele le ts'ebetso ea lifaha tsa makenete, liprotheine tse kopanyang tsa eukaryotic/prokaryotic, mahareng a se nang serum, lisebelisoa tsa setso sa lisele, le tse ling.
Re sebetsa laboratori ea lipatlisiso le nts'etsopele le workshop e hloekileng ea 3,200-square-square-level GMP. Mehaho ena e kenyelletsa sethala sa karohano ea lisele tsa nts'etsopele ea lifaha tsa makenete, boenjiniere ba eukaryotic le prokaryotic protein expression, le nts'etsopele e se nang serum ea serum. Re latela ka bobeli ISO 13485 le ISO 9001 Quality Management System Standards. Ho feta moo, tse ling tsa lihlahisoa tsa rona li na le lifaele tsa FDA DMF.