| Lebitso | Nomoro ea Sengoloa | Tlhaloso | Maemo a polokelo | Shelf Life |
| MaxSortin® CD4 Separation Magnetic Beads | TL-623 | 2 mL bakeng sa 1 × 10⁹ kakaretso ea lisele | 2 - 8 °C | Likhoeli tse 6 |
| MaxSortin® Cell Sorting Buffer | MS-BF100 | 100 ml | 2 - 8 °C | Likhoeli tse 12 |
| MaxSortin® L-Separation Column | MS-CL01 | 1 sengoathoana | 10 - 35 °C | Likhoeli tse 12 |
Mefuta e Sebetsang: Motho
Magnetic Bead Endotoxin:
Ponahalo ea Magnetic Bead: Mokelikeli o sootho
MaxSortin® CD4 Separation Kit e ka sebelisoa ho hlophisa lisele tsa CD4+ T tsa motho. Ka ho kenya li-nanoscale CD4 karohano ea lifaha tsa makenete ka lisele, ho hlophisoa ha lisele tsa CD4+ T ho ka finyelloa. The nanoscale CD4 karohano lifaha magnetic incubated le peripheral blood mononuclear cells (PBMCs) ebe ka tlas'a karohano ea makenete, e leng se nolofalletsang ho arohana le ho ntlafatsa lisele tsa CD4 + T, ho bapala karolo ea ho hloekisa lisele tsa CD4 + T 'me e sebetsa ho hlahisa lihlahisoa tsa phekolo ea lisele.
1. Emisa hape li-PBMC tsa batho ka har'a buffer ea PBS e nang le 1% human serum albumin (HSA). Nka mohlala bakeng sa ho bala le ho fetisetsa lisele tsa 1 × 10⁷ ka har'a tube ea 1.5 mL EP. Ebe centrifuge ka 1500 rpm metsotso e 5.
2. Lahla matla a phahametseng tlhaho, nka 80 μL ea MaxSortin® Cell Sorting Buffer ho khutlisa lisele, eketsa 20 μL ea lifaha tsa karohano tsa CD4 tsa karohano, kopanya hantle ebe u li beha ka sehatsetsing ka 2 - 8 °C bakeng sa ho incubation ka metsotso e 15.
3. Nka Kholomo ea Karohano ea MaxSortin® L 'me u e behe holim'a sehlopa sa ho hlopha lisele tse entsoeng ke makenete (MACS), ebe u se hlatsoa habeli ka 1 mL ea MaxSortin® Cell Sorting Buffer.
4. Nka sampuli e phethiloeng ho tsoa ka sehatsetsing ka 2 - 8 ° C, eketsa 1 mL ea tharollo ea MACS, centrifuge ka 1500 rpm bakeng sa metsotso ea 5, 'me u lahle supernatant.
5. Eketsa 1 mL ea MaxSortin® Cell Sorting Buffer ho tsosolosa lisele, eketsa sampuli ka har'a kholomo ea karohano. Ka mor'a hore e phalle ka tlhaho, eketsa MaxSortin® Cell Sorting Buffer ka likarolo tse peli, 1 mL nako le nako. Bokella litšila ka har'a tube ea 15 ml ea centrifuge.
6. Ka mor'a hore MaxSortin® Cell Sorting Buffer e phalle ka ntle, tlosa kholomo ea karohano ho tloha ho mofuta oa MACS 'me ue behe ka har'a tube e ncha ea 15 mL centrifuge. Kenya 3 mL ea MaxSortin® Cell Sorting Buffer kholomong ea karohano 'me u sebelise piston e tlang le kholomo ea karohano ho sutumelletsa mokelikeli kantle ka kotloloho.
7. Beha 15 mL centrifuge tube e nang le mokelikeli o bokelitsoeng ka har'a centrifuge e otlolohileng le centrifuge ka 1500 rpm metsotso e 5.
8. Ka mor'a centrifugation, tšela matla a maholo, nka 1 mL ea 1 × Dulbecco's phosphate-buffered saline (DPBS) tharollo ea ho emisa lisele, ho bala lisele le ho tsamaisa cytometry ea ho phalla ha metsi.
Ha u kenya lifaha tsa makenete ka lisele, hoa hlokahala ho li kopanya ka botlalo ho ntlafatsa bokhoni ba ho hlophisa.
