| Magaca | Lambarka Maqaalka | Tilmaamid | Xaaladaha Kaydinta | Nolosha shelf |
| MaxSortin® CD4 Kala soocida Kuul Birlabka ah | TL-623 | 2 ml oo ah 1 × 10⁹ unugyo guud | 2 - 8 °C | 6 bilood |
| MaxSortin® Kala-soocidda Unugyada Buffer | MS-BF100 | 100 ml | 2 - 8 °C | 12 bilood |
| MaxSortin® L-Tixda Kala Tagga | MS-CL01 | 1 gabal | 10 - 35 °C | 12 bilood |
Noocyada falcelinta: Aadanaha
Endotoxin kuul magnetic:
Muuqaalka Birlabka Birlabkadareere bunni ah
Qalabka kala-saarida MaxSortin® CD4 waxa loo isticmaali karaa in lagu kala saaro unugyada CD4+ T ee aadanaha. Iyada oo la dhexgelinayo nanoscale CD4 separation magnetic kuul leh unugyo, kala soocidda unugyada CD4+ T waa la gaari karaa. Nanoscale CD4 separation magnetic beads waxaa lagu dhexdaadiyay unugyada mononuclear blood peripheral blood mononuclear (PBMCs) ka dibna waxaa lagu sakhiray kala soocida magnetic, taas oo awood u siinaysa kala soocida iyo kobcinta unugyada CD4+ T, iyaga oo door ka qaadanaya nadiifinta unugyada CD4+ T waxayna khuseysaa soo saarista alaabta daaweynta unugyada.
1. Ku celi PBMC-yada bini'aadamka ee kaydka PBS ee ka kooban 1% serum albumin (HSA). Qaado muunad tirinta oo ku wareeji 1×10⁷ unugyo ku shub 1.5 mL tuubo EP ah. Ka dibna u qabooji 1500 rpm 5 daqiiqo.
2. Tuur xuubka sare, qaado 80 μL ee MaxSortin® Cell Sorting Buffer si aad dib ugu celiso unugyada, ku dar 20 μL oo CD4 ah kala soocida kuul magnetic, si fiican isku walaaq ka dibna ku rid qaboojiyaha at 2 - 8 ° C si aad u qaboojiso 15 daqiiqo.
3. Qaado MaxSortin® L-Separation Column oo dul saar kala-soocidda unugga magnetic-activated (MACS), oo ku luqluq laba jeer adigoo isticmaalaya 1 mL ee MaxSortin® Cell Sorting Buffer.
4. Ka soo qaad muunadda dhammaysay ku-tiirsanaanta qaboojiyaha 2 - 8 ° C, ku dar 1 ml oo xal MACS ah, sentrifuge at 1500 rpm 5 daqiiqo, oo iska tuur dusha sare.
5. Ku dar 1 ml oo ah MaxSortin® Cell Sorting Buffer si aad dib ugu celiso unugyada, ku dar muunada tiirka kala soocida. Ka dib marka ay si dabiici ah u soo baxdo, ku dar MaxSortin® Kala-soocidda Unugyada laba qaybood, 1 ml mar kasta. Ku ururi wasakhda tuubo centrifuge 15 mL ah.
6. Ka dib marka dhammaan MaxSortin® Unugyada Kala-soocidda Buffer ay soo baxaan, ka saar tiirka kala soocida kala-soociyaha MACS oo ku rid tuubo kale oo 15 mL sentifuge ah. Ku dar 3 ml oo ah MaxSortin® Unug Kala soocida tiirka kala soocida oo isticmaal piston-ka la socota tiirka kala soocida si aad dareeraha toos ugu riixdo.
7. Geli tuubada 15 mL centrifuge oo ay ku jiraan dareeraha la ururiyay galka sentrifuge jiifa iyo sentrifuge 1500 rpm ilaa 5 daqiiqo.
8. Ka dib markii centrifugation, ku shub supernatant, qaado 1 mL of 1 × Dulbecco ee fosfooraska-buffered saline (DPBS) xal si ay dib u celiso unugyada, tiriso unugyada iyo qabashada socodka ogaanshaha cytometry.
Marka lagu dhejiyo kuul magnetka leh unugyo, waxaa lagama maarmaan ah in si fiican loo qaso si loo hagaajiyo waxtarka kala-soocidda.
