| Magaca | Lambarka Maqaalka | Tilmaamid | Xaaladaha Kaydinta | Nolosha shelf |
| MaxSortin® CD8 Kala soocida Kuul Birlabka ah | TL-624 | 2 ml oo ah 1 × 10⁹ unugyo guud | 2 - 8 °C | 6 bilood |
| MaxSortin® Kala-soocidda Unugyada Buffer | MS-BF100 | 100 ml | 2 - 8 °C | 12 bilood |
| MaxSortin® L-Tixda Kala Tagga | MS-CL01 | 1 gabal | 10 - 35 °C | 12 bilood |
Noocyada falcelinta: Aadanaha
Endotoxin kuul magnetic:
Muuqaalka Birlabka Birlabkadareere bunni ah
Qalabka kala saarida MaxSortin® CD8 waxa loo isticmaali karaa in lagu kala saaro unugyada CD8+T ee bini'aadamka. Iyada oo la dhexgelinayo nanoscale CD8 kala soocida magnetic kuul leh unugyo, kala soocida unugyada CD8+ T waa la gaari karaa. Nanoscale CD8 separation magnetic kuul waxaa lagu daray unugyada mononuclear dhiigga durugsan (PBMCs) ka dibna la hoos galiyay kala soocidda magnetic, taas oo awood u kala soocidda iyo kobcinta unugyada CD8+ T, ka ciyaaraya door nadiifinta CD8+ T unugyada iyo waa lagu dabaqi karaa in wax soo saarka ee daaweynta unugyada.
1. Ku celi PBMC-yada bini'aadamka ee kaydka PBS ee ka kooban 1% serum albumin (HSA). Qaado muunad tirinta oo ku wareeji 1×10⁷ unugyo ku shub 1.5 mL tuubo EP ah. Ka dibna u qabooji 1500 rpm 5 daqiiqo.
2. Tuur xuubka sare, qaado 80 μL ee MaxSortin® Cell Sorting Buffer si aad dib ugu celiso unugyada, ku dar 20 μL oo CD8 kala soocida kuul magnetka, si fiican isku walaaq ka dibna ku rid qaboojiyaha at 2 - 8 ° C si aad u qaboojiso 15 daqiiqo.
3. Qaado MaxSortin® L-Separation Column oo dul saar kala-soocidda unugga magnetic-activated (MACS), oo ku luqluq laba jeer adigoo isticmaalaya 1 mL ee MaxSortin® Cell Sorting Buffer.
4. Ka soo qaad muunadda dhammaysay ku-tiirsanaanta qaboojiyaha 2 - 8 ° C, ku dar 1 ml oo ah MaxSortin® Cell Sorting Buffer, sentrifuge at 1500 rpm 5 daqiiqo, oo iska tuur tamarta sare.
5. Ku dar 1 ml oo ah MaxSortin® Cell Sorting Buffer si aad dib ugu celiso unugyada, ku dar muunada tiirka kala soocida. Ka dib marka ay si dabiici ah u soo baxdo, ku dar MaxSortin® Kala-soocidda Unugyada laba qaybood, 1 ml mar kasta. Ku ururi wasakhda tuubo centrifuge 15 mL ah.
6. Ka dib marka dhammaan MaxSortin® Unugyada Kala-soocidda Buffer ay soo baxaan, ka saar tiirka kala soocida kala-soociyaha MACS oo ku rid tuubo kale oo 15 mL sentifuge ah. Ku dar 3 ml oo ah MaxSortin® Unug Kala soocida tiirka kala soocida oo isticmaal piston-ka la socota tiirka kala soocida si aad dareeraha toos ugu riixdo.
7. Geli tuubada 15 mL centrifuge oo ay ku jiraan dareeraha la ururiyay galka sentrifuge jiifa iyo sentrifuge 1500 rpm ilaa 5 daqiiqo.
8. Ka dib markii centrifugation, ku shub supernatant, qaado 1 mL of 1 × Dulbecco ee fosfooraska-buffered saline (DPBS) xal si ay dib u celiso unugyada, tiriso unugyada iyo qabashada socodka ogaanshaha cytometry.
1. Markaad ku dhexjirto kuul birlab ah oo leh unugyo, waxaa lagama maarmaan ah in si fiican loo qaso si loo hagaajiyo waxtarka kala-soocidda.
2. Tiirka kaydinta iyo kala soocida ee ku jira xirmadan waxa loo isticmaali karaa tijaabada hore. Haddii hawlgallo tijaabo ah oo dheeraad ah ay ku lug leeyihiin, fadlan soo iibso MaxSortin® Kala-soocidda Unugyada Buffer (Lambarka Maqaalka: MS-BF100) iyo MaxSortin® L-Separation Column (Lambarka Maqaalka: MS-CL01) si gaar ah.
Alaabtan waxa kaliya oo lagu dabaqi karaa dhaqanka unugyada vitro si toos ahna looma isticmaali karo daawaynta kiliinikada.
