| Zita | Nhamba Yechinyorwa | Tsanangudzo | Storage Conditions | Sherufu Hupenyu |
| MaxSortin® CD4 Separation Magnetic Beads | TL-623 | 2 mL ye1 × 10⁹ yakazara maseru | 2 - 8 °C | 6 mwedzi |
| MaxSortin® Cell Sorting Buffer | MS-BF100 | 100 mL | 2 - 8 °C | 12 mwedzi |
| MaxSortin® L-Kuparadzanisa Column | MS-CL01 | 1 chidimbu | 10 - 35 °C | 12 mwedzi |
Reactive Species: Munhu
Magnetic Bead Endotoxin:
Magnetic Bead Kuonekwa: Brown mvura
Iyo MaxSortin® CD4 Separation Kit inogona kushandiswa kugadzirisa masero eCD4+ T emunhu. Kuburikidza nekuisa nanoscale CD4 kupatsanurwa magineti mabedhi nemaseru, kurongwa kweCD4+ T masero kunogona kuwanikwa. Iyo nanoscale CD4 kupatsanurwa magineti mabedhi anoiswa neperipheral ropa mononuclear masero (PBMCs) uye obva aiswa kune magineti kupatsanurwa, izvo zvinoita kuti kupatsanurwa uye kupfumisa kweCD4 + T masero, ichiita basa rekuchenesa CD4 + T masero uye inoshanda pakugadzirwa kwesero therapy zvigadzirwa.
1. Dzorera zvakare maPBMC evanhu muPBS buffer ine 1% human serum albumin (HSA). Tora sampuli yekuverenga uye kuendesa 1 × 10⁷ masero mu 1.5 mL EP chubhu. Zvadaro centrifuge pa 1500 rpm kwemaminitsi mashanu.
2. Rasa mashura, tora 80 μL yeMaxSortin® Cell Sorting Buffer kuti umisezve maseru, wedzera 20 μL yeCD4 separation magnetic beads, sanganisa zvakanaka wozoaisa mufiriji pa 2 - 8 °C for incubation kwemaminetsi gumi nemashanu.
3. Tora MaxSortin® L-Separation Column woiisa pamagetsi-activated cell sorting (MACS) sorter, woigeza kaviri ne1 mL yeMaxSortin® Cell Sorting Buffer.
4. Tora muenzaniso wapedza kuvharwa kunze kwefiriji pa 2 - 8 ° C, wedzera 1 mL yeMAC solution, centrifuge pa 1500 rpm kwemaminitsi mashanu, uye urase iyo supernatant.
5. Wedzera 1 mL yeMaxSortin® Cell Sorting Buffer kuti udzorere masero, wedzera sampuli mumutsara wekuparadzanisa. Mushure mekuyerera kwega, wedzera MaxSortin® Cell Sorting Buffer muzvikamu zviviri, 1 mL nguva yega yega. Unganidza tsvina mune 15 mL centrifuge chubhu.
6. Mushure mokunge MaxSortin® Cell Sorting Buffer yayerera kunze, bvisa chikamu chekuparadzanisa kubva paMACS sorter uye uise mune imwe itsva 15 mL centrifuge chubhu. Wedzera 3 mL yeMaxSortin® Cell Sorting Buffer muchikamu chekuparadzanisa uye shandisa piston inouya nekoramu yekuparadzanisa kusundira mvura kunze zvakananga.
7. Isa 15 mL centrifuge chubhu ine mvura yakaunganidzwa muhorizontal centrifuge uye centrifuge pa 1500 rpm kwemaminitsi mashanu.
8. Mushure mekuita centrifugation, dururira iyo supernatant, tora 1 mL ye 1 × Dulbecco's phosphate-buffered saline (DPBS) mhinduro yekumisazve masero, kuverenga masero uye kuitisa kuyerera kwe cytometry kuona.
Paunenge uchiisa magineti mabedhi nemasero, zvinodikanwa kuti uvasanganise zvakakwana kuti uvandudze kugona kwekuronga.
