Le Siamupini Toe faaleleia i Siosiomaga Maualuluga! O le T&L Biotechnology ua fa'alauiloa aloa'ia le fa'atupuina fou o le Thermotable bFGF Protein.
Basic Fibroblast Growth Factor (bFGF), o se sui o le fibroblast growth factor family, e fa'aosoina ala fa'aliliuina fa'ailoga e ala i le fusia ma fa'agaoioia o fa'afofoga ile targeT Cell luga. bFGF faʻatonutonuina le faʻateleina o ituaiga sela eseese, e aofia ai sela endothelial, neurons, chondrocytes, sela maso lamolemole, melanocytes, ma adipocytes. E le gata i lea, o le bFGF o loʻo aʻafia i faiga faʻapitoa e pei o le faʻafouina o le tagata lava ia, faʻavasegaina, atinaʻe, toe faʻaleleia, ma toe faʻafouina. I le avea ai o se mea taua mo le tuputupu aʻe o sela, o le bFGF e faʻaaogaina lautele i le aganuu in vitro o ituaiga sela eseese.
O suʻesuʻega e faʻaalia ai o le bFGF e le mautonu i lalo o tulaga faʻaletino, ma le afa-ola e tusa ma le 8 itula i aganuʻu sela mamame. O le maualalo o le mafanafana ma le afa puupuu o le ola o le bFGF e oʻo atu ai i luʻitau i le aganuʻu sela, e manaʻomia ai le faʻaopoopoina o mea faʻatupu soo poʻo le suia o aso taʻitasi. Ole polotini bFGF thermostable, fa'ainisinia e ala i suiga ole bFGF fa'alenatura, e fa'afeiloa'i ai lenei lu'itau. O lo'o fa'atumauina le gaioioiga malosi fa'ale-natura a le bFGF fa'alenatura a'o ausia le fa'aleleia atili o le fa'amautuina o le vevela, fa'atumauina le sa'o fa'atulagaina ma le gaioiina o porotini i le lautele o le vevela.
Embryonic tagata Stem Cells (hESCs) ma induced pluripotent stem cells (iPSCs) e iai le malosi e mafai ona fa'aeseese i ituaiga somatic cell uma. I le taimi o aganuʻu in vitro o hESCs ma iPSCs, e manaʻomia le bFGF mo le faʻatumauina o le tele o le cell cell. O su'esu'ega o lo'o fa'aalia ai o le hESC na fa'atupuina ma le bFGF e mafai ona fa'afefeteina e fausia ni kolone e le'i fa'avasegaina vave (Fig. 1A) ma fa'aalia ai le fa'atuputeleina o fua fa'atatau i le aganu'u umi (Fig. 1B). O isi suʻesuʻega e faʻaalia ai o le bFGF e mafai ona faʻafefeteina e faʻamalosia ai le faʻateleina o le hESC aʻo lagolagoina le faʻaaliga o faʻailoga pluripotency (faʻataʻitaʻiga, OCT4, SOX2, KLF4), faʻamaonia lona gafatia e faʻasaoina le hESC pluripotency-o se vaega taua mo le faʻaogaina o aganuu.
A: Tulaga tuputupu a'e o hESC i le Aso 2 i lalo o tulaga fa'aleaganu'u e leai ni fafaga fa'atasi ai ma suiga eseese o le bFGF.
B: Fa'atusatusaga o fua faatatau o le tuputupu a'e o sela i le taimi fa'aauau aganu'u ma le WT-bFGF (ituaiga vao) po'o le TS-bFGF (thermostable) i lalo ole tulaga e leai ni fafaga.
Fa'aliga oloa
Recombinant Human bFGF Porotini (Avevela)
Oloa Fa'ailoga
- Maumea
- maualuga mama (>90%)
- Gaoioiga Maualuga
- Endotoxin maualalo (
- Fa'amama
- Mycoplasma-Sa'oloto
Galuega Fa'atino
fa'ata'ita'iga
A fa'aopoopo i le iPSC aganu'u ala, e mafai ona fa'atino le suiga o le ala ile 2 aso.
E pei ona faʻaalia i le Ata 5, T&L's Recombinant Human bFGF Protein (GMP-TL901) ma Thermostable bFGF na faʻaaogaina i le AMMS® Pluripotent Stem Cell Medium (AS-35) mo le aganuu iPSC. I le ala fa'aopoopo i le bFGF e mafai ona fa'afefeteina, o aso ta'itasi po'o isi aso uma suiga fa'aleaogaina ua matua fa'aleleia ai numera ma lapopo'a fa'atusatusa ile bFGF masani. O le mea moni, o le fa'aogaina o le cell e le'i fa'aalia se eseesega tele i le va o aso uma ma isi aso uma fa'atasi ai ma le bFGF fa'amautu, fa'amaonia lona talafeagai mo le aganu'u iPSC taugofie.
E fiafia e fa'ata'ita'i le tatou New-Generation Thermotable bFGF Protein?
Fa'afeso'ota'i i matou i le aso e talosagaina ai se fa'ata'ita'iga e leai se totogi ma maua fa'amanuiaga mo au su'esu'ega!
T&L Biotechnology Co., Ltd. e taulaʻi i le suʻesuʻeina ma le atinaʻeina o mea mataʻutia i luga, GMP-grade ma reagents mo sela ma Togafitiga Gene (CGT). Matou te tuuto atu i le tuʻuina atu o fofo atoa mo tagata faʻatau CGT e ala i la matou oloa atoatoa ma ofo tautua. O a tatou oloa e aofia ai vavaeeseina o sela ma activation maneta lopa, eukaryotic/prokaryotic recombinant proteins, serum-free medium, cell culture kits, ma isi mea.
Matou te fa'agaioia se falesu'esu'e ma atina'e falesu'esu'e ma se falea'oga mama 3,200 mita sikuea mita GMP. O nei nofoaga e aofia ai fa'avae mo le vavae'esega o sela maneta le atina'e, eukaryotic ma le prokaryotic protein expression inisinia, ma serum-free medium development. Matou te tausisia uma le ISO 13485 ma ISO 9001 Quality Management System Standards. E le gata i lea, o nisi oa matou oloa o loʻo i ai faʻamaumauga a le FDA DMF.