Nyampinga wo Gusana Mubihe Byinshi-Ubushyuhe! T&L Ikoranabuhanga ryibinyabuzima ryatangije kumugaragaro Ibisekuru bishya bya Thermostable bFGF Protein
Iterambere ryibanze rya Fibroblast (bFGF), umwe mubagize umuryango ukura wa fibroblast, utera inzira yerekana inzira ya transduction muguhuza no gukora reseptors kuri targeT Akagari Ubuso. bFGF igenga ikwirakwizwa ryubwoko butandukanye bwingirabuzimafatizo, harimo selile endothelia selile, neuron, chondrocytes, selile yimitsi yoroshye, melanocytes, na adipocytes. Byongeye kandi, bFGF igira uruhare mubikorwa byibinyabuzima nka selile kwiyubaka, gutandukanya, iterambere, gusana ingirangingo, no kuvugurura. Nkikintu gikomeye cyiterambere ryimikorere, bFGF ikoreshwa cyane mumico ya vitro yubwoko butandukanye.
Ubushakashatsi bwerekana ko bFGF idahindagurika mubihe byimiterere yumubiri, hamwe nigice cyubuzima bwamasaha agera kuri 8 mumico y’inyamabere. Ubushyuhe buke bwumuriro hamwe nigihe gito cyubuzima bwa bFGF biganisha kubibazo mumico yutugari, bisaba ko hongerwaho ibintu byinshi byo gukura cyangwa gusimbuza buri munsi. Poroteyine yubushyuhe bwa bFGF, yakozwe muburyo bwo guhindura bFGF karemano, ikemura iki kibazo. Igumana ibikorwa byibinyabuzima bya bFGF karemano mugihe bigera ku kongera ubushyuhe bwumuriro, bikomeza uburinganire bwimiterere nibikorwa bya poroteyine mubushuhe bugari.
Urusoro rw'umuntu Akagaris (HESCs) hamwe na pluripotent stem selile (iPSCs) bifite ubushobozi bwo gutandukanya ubwoko bwose bwa selile. Mugihe mumico ya vitro ya HESCs na iPSCs, bFGF ningirakamaro mugukomeza ingirabuzimafatizo. Ubushakashatsi bwerekana ko HESCs ifite umuco wa bFGF ikora ubukoroni butandukanye (Igicapo 1A) kandi ikagaragaza ubwiyongere bukabije bwikwirakwizwa mugihe cyumuco muremure (Ishusho 1B). Ubushakashatsi bwakozwe burerekana ko bFGF iteza imbere ikwirakwizwa rya HESC mugihe ikomeza kwerekana ibimenyetso bya pluripotency (urugero, OCT4, SOX2, KLF4), byemeza ubushobozi bwayo bwo kubungabunga pluripotensiya ya HESC - ikintu cyingenzi mubikorwa byogukoresha umuco.
Igisubizo: Iterambere ryimiterere ya HESCs kumunsi wa 2 mubihe byumuco utagira ibiryo hamwe na bFGF zitandukanye.
B: Kugereranya umuvuduko wubwiyongere bwakagari mugihe cyumuco uhoraho hamwe na WT-bFGF (ubwoko bwishyamba) cyangwa TS-bFGF (thermostable) mubihe bitarimo ibiryo.
Gutangiza ibicuruzwa
Recombinant Umuntu bFGF Poroteyine (Ubushyuhe buhamye)
Ibiranga ibicuruzwa
- Ubushuhe
- Isuku ryinshi (> 90%)
- Igikorwa Cyinshi
- Endotoxine yo hasi (
- Sterile
- Mycoplasma-Yubusa
Imikorere y'ibicuruzwa
Gusaba
Iyo wongeyeho umuco wa iPSC urwego, gusimbuza hagati birashobora gukorwa buri minsi 2.
Nkuko bigaragara ku gishushanyo cya 5, T & L's Recombinant Human bFGF Protein (GMP-TL901) na Thermostable bFGF yakoreshejwe kuri AMMS® Pluripotent Stem Cell Medium (AS-35) kumuco wa iPSC. Hagati hiyongereyeho ubushyuhe bwa bFGF, burimunsi cyangwa burimunsi-iyindi minsi yo hagati yahinduye cyane umubare wabakoloni nubunini ugereranije na bFGF isanzwe. Ikigaragara ni uko ubushobozi bwimikorere bwakagari bwerekanye ko nta tandukaniro rinini riri hagati ya buri munsi na buri munsi-protocole hamwe na bFGF ya thermostable bFGF, yemeza ko ikwiranye n'umuco wa iPSC uhendutse.
Ushishikajwe no kugerageza Ibisekuru bishya bya Thermostable bFGF Protein?
Twandikire uyumunsi kugirango dusabe icyitegererezo cyubusa kandi wibonere inyungu kubushakashatsi bwawe!
T&L Biotechnology Co., Ltd. yibanze ku bushakashatsi niterambere ryiterambere, ibikoresho fatizo byo mu rwego rwa GMP na reagent ya selile na Ubuvuzi bwa Gene (CGT). Twiyemeje gutanga ibisubizo byuzuye kubakiriya ba CGT binyuze mubicuruzwa byuzuye hamwe na serivisi zitangwa. Ibicuruzwa byacu birimo gutandukanya selile na Gukoresha amashanyarazi, eukaryotic / prokaryotic recombinant proteyine, serumu idafite imiti, ibikoresho byumuco, n'ibindi.
Dukora laboratoire yubushakashatsi niterambere hamwe na metero 3.200-ya metero kare ya GMP. Ibi bikoresho birimo urubuga rwo gutandukanya ingirabuzimafatizo ya magneti, iterambere rya eukaryotic na prokaryotic protein imvugo yubuhanga, hamwe niterambere ridafite serumu. Twubahiriza ibipimo byombi ISO 13485 na ISO 9001. Byongeye kandi, bimwe mubicuruzwa byacu bifite dosiye ya FDA DMF.