| Izina | Umubare Umubare | Ibisobanuro | Ububiko | Ubuzima bwa Shelf |
| MaxSortin® CD4 Gutandukanya Amasaro ya Magnetique | TL-623 | 2 mL kuri 1 × 10⁹ selile zose | 2 - 8 ° C. | Amezi 6 |
| MaxSortin® Akagari ka Sorting Buffer | MS-BF100 | 100 mL | 2 - 8 ° C. | Amezi 12 |
| MaxSortin® L-Gutandukanya Inkingi | MS-CL01 | Igice 1 | 10 - 35 ° C. | Amezi 12 |
Ubwoko Bwiza: Umuntu
Amashanyarazi ya Magnetic Endotoxin:
Kugaragara kw'isaro rya Magnetique: Amazi yijimye
Igikoresho cyo Gutandukanya MaxSortin® CD4 gishobora gukoreshwa mugutandukanya selile CD4 + T. Binyuze muri incubasi ya nanoscale CD4 itandukanya amasaro ya magneti hamwe na selile, gutondekanya selile CD4 + T birashobora kugerwaho. Nanoscale CD4 itandukanya amasaro ya magnetiki yinjizwemo na selile periferique mononuclear selile (PBMCs) hanyuma igakorerwa gutandukana kwa magneti, ituma gutandukanya no gutunganyiriza ingirabuzimafatizo za CD4 + T, bigira uruhare mukweza selile CD4 + T kandi bikoreshwa mugukora ibicuruzwa bivura selile.
1. Hagarika PBMCs zabantu muri buffer ya PBS irimo 1% ya serumu yumuntu (HSA). Fata icyitegererezo cyo kubara no kohereza 1 × 10⁷ selile muri 1.5 mL ya EP. Noneho centrifuge kuri 1500 rpm kuminota 5.
2. Hagarika ndengakamere, fata 80 μL ya MaxSortin® Cell Sorting Buffer kugirango uhagarike selile, ongeramo 20 μL ya CD4 itandukanya amashanyarazi ya magnetiki, uvange neza hanyuma ubishyire muri firigo kuri 2 - 8 ° C kugirango ube wongeyeho iminota 15.
3. Fata Inkingi ya MaxSortin® L-uyishyire kumurongo wa magnetiki ukoreshwa na selile (MACS), hanyuma ubyoze kabiri hamwe na mL 1 ya MaxSortin® Sorting Buffer.
4. Fata icyitegererezo cyarangije incububasi muri firigo kuri 2 - 8 ° C, ongeramo 1 mL yumuti wa MACS, centrifuge saa 1500 rpm muminota 5, hanyuma ujugunye ndengakamere.
5. Ongeramo 1 mL ya MaxSortin® Sorting Buffer kugirango uhagarike selile, ongeraho icyitegererezo mumurongo wo gutandukana. Nyuma yo gusohoka bisanzwe, ongeramo MaxSortin® Cell Sorting Buffer mubice bibiri, 1 mL buri gihe. Kusanya imyanda muri 15 mL ya centrifuge.
6. Nyuma yuko byose bya MaxSortin® Cell Sorting Buffer imaze gusohoka, kura inkingi yo gutandukana nu muti wa MACS hanyuma uyishyire muyindi miyoboro 15 mL ya centrifuge. Ongeramo 3 mL ya MaxSortin® Cell Sorting Buffer mu nkingi yo gutandukana hanyuma ukoreshe piston ije ifite inkingi yo gutandukana kugirango usunike amazi hanze.
7. Shira umuyoboro wa mL 15 mL urimo amazi yakusanyirijwe muri horizontal centrifuge na centrifuge kuri 1500 rpm muminota 5.
8.
Iyo ushizemo amasaro ya magnetique hamwe na selile, ni ngombwa kuyivanga neza kugirango utezimbere imikorere.
