0102030405
GMP-TL101 Kurwanya Umuntu CD3 mAb Kumenyekanisha Antigen, kwanduza ibimenyetso no gukora T selile
Incamake y'ibicuruzwa
Anti-muntu CD3 mAb (GMP TL101) yakozwe kugirango yorohereze kumenyekanisha antigen, kwanduza ibimenyetso, no gukora T selile. Iyi antibody ya monoclonal nikintu cyingenzi mubushakashatsi ndetse nubuvuzi bukoreshwa na T selile manipulation.
Ibintu by'ingenzi
Trans Transduction ya signal: Ihuza neza kumenyekanisha antigen no guhererekanya ibimenyetso, byingenzi mugukora T selile.
Manufacturing GMP Gukora: Yakozwe mubihe bikomeye bya GMP kugirango ubuziranenge bwibicuruzwa kandi byororoke.
● Umwihariko wo hejuru: Umwihariko wa CD3, ukemeza neza intego ningaruka ntoya.
Appl Ikoreshwa ryagutse: Birakwiriye mubikorwa bitandukanye, harimo cytometrike itemba, umuco w'akagari, n'ubushakashatsi bwo kuvura.
Porogaramu
Research Ubushakashatsi bwikingira: Ibyingenzi mukwiga T selile reseptor (TCR) amarenga no gukora inzira.
Development Iterambere ryubuvuzi: Imfashanyo mugutezimbere imiti ya T, harimo na CAR-T ivura.
Studies Ubuvuzi bwa Clinical: Bikwiranye na protocole yubushakashatsi bwamavuriro busaba antibodiyite zujuje ubuziranenge, zubahiriza GMP.
Ibisobanuro hamwe no gupakira
Ibisobanuro:
Kuboneka mubunini bwinshi kugirango uhuze ibikenewe bitandukanye byubushakashatsi: 100μg, 500μg, na 1mg.
Ibipimo byujuje ubuziranenge byemeza guhuzagurika no kwizerwa.
Gupakira:
Itangwa nkamazi meza cyangwa ifu ya lyofilize kugirango byoroshye gukoreshwa no kubika neza.
Gipakirwa kugirango ubungabunge ubunyangamugayo no gukora neza mugihe cyo gutwara no gutwara.
Kubika no Guhagarara
Ububiko
Kugira ngo ukoreshe igihe gito, bika kuri 2-8 ° C.
Kubika igihe kirekire, komeza kuri -20 ° C kugirango ubungabunge antibody nibikorwa.
Irinde kuzenguruka inshuro nyinshi kugirango wirinde kwangirika.
Igihagararo
Igumana ituze mubihe byububiko byasabwe, byemeza imikorere yizewe mubushakashatsi.
Yashizweho kugirango irambe, yemeza neza igihe kirekire.
Amabwiriza yo Gukoresha
Koresha antibody muri buffer cyangwa uburyo bukwiye mbere yo gukoresha.
Tegura kandi ukoreshe igisubizo ukurikije ibisabwa byubushakashatsi kubisubizo nyabyo kandi byororoka.
Kurikiza protocole ijyanye no gusaba kwawe, yaba flux cytometrie, immunohistochemie, cyangwa assay imikorere.
Icyitonderwa
• Iki gicuruzwa kigenewe gusa gukoresha ubushakashatsi kandi ntikibereye kwisuzumisha kwa muganga cyangwa kuvura.
• Koresha antibody ukurikije amabwiriza yatanzwe kugirango ukomeze ibikorwa byayo n'ubunyangamugayo.
• Kubibazo cyangwa ibibazo, nyamuneka hamagara itsinda ryacu rishinzwe ubufasha bwa tekiniki kugirango tugufashe.
• Hindura ubushakashatsi bwawe hamwe na antibodies nyinshi kandi zizewe. Kumakuru arambuye nibisobanuro, sura urubuga.
| Antibodies za Monoclonal | |
| Porogaramu | Uburyo bukemuka: birasabwa kwibanda kuri 500 ng / mL, munsi ya T selile ishobora gukora kandi CD3⁺CD56⁺T selile ishobora kwagurwa kuburyo bugaragara. |
| Igikorwa cyibinyabuzima | Igipimo cyo guhuza ingirabuzimafatizo za Jurkat ntigishobora kuba munsi ya 90% |
| Uburyo bwo gutwikira: | 10 mL ya buffer ya PBS (5μg / mL anti-muntu CD3) ishyirwa mumashanyarazi ya T75 kuri 4 ° C ijoro ryose. Nyamuneka kura PBS hanyuma ukarabe flask hamwe na saline witonze inshuro eshatu mbere yo kuyikoresha. |
| Endotoxin | |
| Ikirangantego | CHO selile |
| Gutegura | Yatanzwe nkigisubizo cya 0.22 mm muri PBS, PH 7.4 |
| Isuku ： | Poroteyine Yeza mumico y'akagari ndengakamere |
| QC Kugerageza Ubuziranenge | > 95% nkuko byagenwe na SDS-PAGE |
| Guhagarara & Kubika | Amezi 24 kuri 2 ℃ kugeza 8 ℃. Irinde gusubiramo inshuro nyinshi |
| Igikumwe | Ibisobanuro bya dosiye |
 | GMP-TL101_SDS.pdf |
| GMP-TL101_Urupapuro rwerekana ibicuruzwa.pdf |