| Dzina | Nambala Yankhani | Kufotokozera | Zosungirako | Shelf Life |
| MaxSortin® CD4 Separation Magnetic Mikanda | Mtengo wa TL-623 | 2 mL kwa 1 × 10⁹ maselo onse | 2-8 ° C | 6 miyezi |
| MaxSortin® Cell Sorting Buffer | Chithunzi cha MS-BF100 | 100 ml | 2-8 ° C | Miyezi 12 |
| Mzere Wopatukana wa MaxSortin® L | Chithunzi cha MS-CL01 | 1 chidutswa | 10-35 ° C | Miyezi 12 |
Mitundu Yokhazikika: Munthu
Magnetic Bead Endotoxin
Mawonekedwe a Magnetic Bead: Madzi a bulauni
The MaxSortin® CD4 Separation Kit ikhoza kugwiritsidwa ntchito kusanja ma CD4+ T aumunthu. Kupyolera mu incubating nanoscale CD4 kupatukana maginito mikanda ndi maselo, kusanja ma CD4+ T maselo angapezeke. The nanoscale CD4 kupatukana maginito mikanda ndi incubated ndi zotumphukira magazi mononuclear maselo (PBMCs) ndiyeno pansi pa maginito kulekana, zomwe zimathandiza kulekana ndi kulemeretsa CD4 + T maselo, kuchita mbali mu kuyeretsa CD4 + T maselo ndi ntchito kupanga mankhwala mankhwala mankhwala.
1. Kuyimitsanso ma PBMC a anthu mu PBS buffer yomwe ili ndi 1% human serum albumin (HSA). Tengani chitsanzo chowerengera ndikusamutsa 1 × 10⁷ maselo mu chubu cha 1.5 mL EP. Kenako centrifuge pa 1500 rpm kwa mphindi 5.
2. Tayani zamphamvu kwambiri, tengani 80 μL ya MaxSortin® Cell Sorting Buffer kuti muyimitsenso ma cell, onjezerani 20 μL wa CD4 kupatukana mikanda ya maginito, sakanizani bwino ndiyeno muyike mufiriji pa 2 - 8 °C kwa ma incubation kwa mphindi khumi ndi zisanu.
3. Tengani MaxSortin® L-Separation Column ndikuyiyika pa chosankha cha maginito (MACS), ndikutsuka kawiri ndi 1 mL ya MaxSortin® Cell Sorting Buffer.
4. Tengani chitsanzo chomwe chatsiriza kutulutsa mufiriji pa 2 - 8 ° C, onjezerani 1 mL ya njira ya MACS, centrifuge pa 1500 rpm kwa mphindi 5, ndikutaya supernatant.
5. Onjezani 1 mL ya MaxSortin® Cell Sorting Buffer kuti muyimitsenso maselo, onjezerani chitsanzo mu gawo lolekanitsa. Ikatuluka mwachibadwa, onjezani MaxSortin® Cell Sorting Buffer m'magawo awiri, 1 mL nthawi iliyonse. Sungani zotayira mu chubu cha 15mL centrifuge.
6. Pambuyo pa MaxSortin® Cell Sorting Buffer yatuluka, chotsani gawo lolekanitsa kuchokera ku chosankha cha MACS ndikuchiyika mu chubu china chatsopano cha 15 mL centrifuge. Onjezani 3 mL ya MaxSortin® Cell Sorting Buffer mugawo lolekanitsa ndipo gwiritsani ntchito pistoni yomwe imabwera ndi gawo lolekanitsa kukankhira madziwo mwachindunji.
7. Ikani chubu cha 15 mL centrifuge chomwe chili ndi madzi osonkhanitsidwa mu centrifuge yopingasa ndi centrifuge pa 1500 rpm kwa mphindi zisanu.
8. Pambuyo pa centrifugation, tsanulirani mphamvu yapamwamba, tengani 1 mL ya 1 × Dulbecco's phosphate-buffered saline (DPBS) yankho kuti muyimitsenso maselo, kuwerengera maselo ndi kuyendetsa kutuluka kwa cytometry.
Pamene incubating ndi maginito mikanda ndi maselo, m`pofunika kusakaniza bwinobwino kusintha kusanja dzuwa.
