New Product Launch: Recombinant Human TPO Protein—A Breakthrough in Hematopoietic Regulation and Stem Cell Culture Innovation!

2025-05-20

Thrombopoietin (Tpo), also known as Megakaryocyte Growth and Development Factor (MGDF), is a cytokine that regulates megakaryocyte production and platelet generation. TPO acts early in megakaryocyte lineage commitment, promoting the proliferation of megakaryocyte progenitor cells and increasing megakaryocyte ploidy. However, TPO does not influence platelet release from mature megakaryocytes [1].

TPO plays a pivotal role in hematopoietic Stem Cell (HSC) regulation (Figure 1). Studies reveal that TPO signaling in HSCs maintains stem cell quiescence to preserve their longevity or drives HSC expansion during critical events (e.g., post-transplantation). In both scenarios, TPO modulates cell cycle transitions. The TPO-Mpl signaling pathway impacts mitochondrial metabolism, and this metabolic regulation may influence HSC lineage differentiation. Furthermore, TPO signaling stimulates HSC self-renewal, highlighting its therapeutic potential for treating hematopoietic disorders characterized by impaired stem cell function, such as aplastic anemia, through TPO-mimetic drug development.

Figure 1. Schematic diagram of TPO functions in HSCs [2].

In HSC ex vivo culture, TPO enhances cell expansion. While TPO alone minimally impacts proliferation in single-cell culture systems, its combination with SCF, IL-3, and IL-6 significantly boosts HSC clonal growth (Table 1). Recent advances have further optimized cytokine cocktail formulations incorporating TPO for improved HSC culture outcomes [4,5].

Table 1. Effects of TPO on HSC colony formation in vitro [3].

T&L Biotechnology Proudly Introduces: Recombinant Human TPO Protein (TL-650)

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TL-650 (50μg)

Product Features

High purity
Exceptional bioactivity
Low endotoxin
Sterile, mycoplasma-free

Product Performance Validation

Figure 2. SDS-PAGE analysis under reducing conditions confirms >90% purity.

Figure 3. Mo7e cell proliferation assay: T&L product shows >5x activity vs. Imported competitors.

References

[1] de Graaf CA, Metcalf D. Thrombopoietin and hematopoietic stem cells. Cell Cycle. 2011;10(10):1582-9. doi:10.4161/cc.10.10.15619.

[2] Nakamura-Ishizu A, Suda T. Multifaceted roles of thrombopoietin in hematopoietic stem cell regulation. Ann N Y Acad Sci. 2020;1466(1):51-58. doi:10.1111/nyas.14169.

[3] Sitnicka E, et al. The effect of thrombopoietin on murine hematopoietic stem cells. Blood. 1996;87:4998-5005. PMID:8652812.

[4] Pineault N, et al. Cytokine effects on cord blood CD34+ expansion. Cytotherapy. 2010;12:467-80.

[5] Ohmizono Y, et al. TPO synergizes with SCF/flt3 ligand in cord blood HSC expansion. Leukemia. 1997;11:524-30. doi:10.1038/sj.leu.2400588.