| Ingoa | Tau Tuhinga | Whakatakotoranga | Nga Tikanga Rokiroki | Te Ora Papa |
| MaxSortin® CD4 Wehenga Piira aukume | TL-623 | 2 mL mō te 1×10⁹ ngā pūtau katoa | 2 - 8 °C | 6 marama |
| MaxSortin® Pūtau Kōmaka Buffer | MS-BF100 | 100 mL | 2 - 8 °C | 12 marama |
| MaxSortin® L-Tiwae Wehewehenga | MS-CL01 | 1 wahi | 10 - 35 °C | 12 marama |
Nga momo tauhohenga: Tangata
Endotoxin Bead aukume:
Te ahua o te pirepire aukume: Wai parauri
Ka taea te whakamahi i te MaxSortin® CD4 Separation Kit ki te kōmaka i nga pūtau CD4+ T tangata. Na roto i te incubating nanoscale CD4 wehe pirepire autō ki ngā pūtau, te kōmaka o CD4+ pūtau T e taea te tutuki. Ko te nanoscale CD4 whakawehenga pirepire autō e incubated ki peripheral toto mononuclear pūtau (PBMCs) ka i raro ki te wehenga autō, e taea ai te wehenga me te whakarangatiratanga o CD4 + T pūtau, whai wāhi ki te pure CD4 + T pūtau me te e hāngai ana ki te hanga o ngā hua rongoā pūtau.
1. Whakaorangia nga PBMC tangata i roto i te PBS buffer kei roto te 1% albumin serum tangata (HSA). Tangohia he tauira mo te tatau me te whakawhiti 1×10⁷ pūtau ki roto i te ngongo EP 1.5 mL. Katahi ka whakahiatohia ki te 1500 rpm mo te 5 meneti.
2. Makahia te supernatant, tangohia te 80 μL o MaxSortin® Cell Sorting Buffer hei whakatārewa i ngā pūtau, tāpirihia te 20 μL o ngā pirepire autō whakawehe CD4, uru pai ka waiho ki roto i te pouaka whakamātao i te 2 - 8 °C mo te heki mo te 15 meneti.
3. Tangohia te MaxSortin® L-Separation Column ka tuu ki runga i te komaka-a-whakahohe-a-hiko (MACS), ka horoi kia rua ki te 1 mL o MaxSortin® Cell Sorting Buffer.
4. Tangohia te tauira kua oti te heki i roto i te pouaka whakamātao i te 2 - 8 °C, tāpirihia te 1 mL o te otinga MACS, te centrifuge i te 1500 rpm mo te 5 meneti, ka makahia te supernatant.
5. Taapirihia te 1 mL o MaxSortin® Pūtau Whakatairanga Buffer hei whakatārewa i ngā pūtau, tāpirihia te tauira ki te tīwae wehenga. Whai muri i te rere ki waho, taapirihia te MaxSortin® Pūkoro Kōmaka Buffer kia rua nga wahanga, 1 mL ia wa. Kohia te para ki roto i te ngongo 15 mL centrifuge.
6. I muri i te rere katoa o te MaxSortin® Cell Sorting Buffer, tangohia te pou wehenga mai i te MACS sorter ka tuu ki tetahi atu ngongo centrifuge 15 mL hou. Taapirihia te 3 mL o MaxSortin® Pūtau Whakaraupapa Buffer ki te tīwae wehenga ka whakamahi i te piston ka puta mai me te pou wehenga hei pana tika i te wai ki waho.
7. Whakanohoia te ngongo 15 mL centrifuge kei roto te wai kua kohia ki roto i te centrifuge whakapae me te centrifuge i te 1500 rpm mo te 5 meneti.
8. I muri i te centrifugation, ringihia te supernatant, tangohia 1 mL o 1× Dulbecco's phosphate-buffered saline (DPBS) otinga ki te whakatārewa i nga pūtau, tatau nga pūtau me te whakahaere i te rerenga cytometry.
I te wa e whakapouri ana i nga pirepire aukume ki nga pūtau, me tino whakakotahi kia pai ake te pai o te tohatoha.
