Total Cell Capacity: 10⁷ to 2×10⁹ cells.
Capacity for Labeled Cells: 10⁵ to 10⁸ cells (When separating cells larger than lymphocytes, the capacity of the separation column may decrease).
Void Volume: 1.35 ml.
Reservoir Volume: 7 ml.
Flow Rate: For 1x PBS containing 0.5% BSA (Bovine Serum Albumin): 1.2 - 2.1 ml/min.
Applicable Range: Applicable to samples with a diameter less than 30 μm.
Sterility: The separation columns are individually packaged in a sterile manner.
MaxSortin® L-Separation Columns are used in combination with MaxSortin® series of cell sorting magnetic beads and cell sorting buffers. MaxSortin® L-Separation Columns can gently sort the cells bound by magnetic beads.
Experimental Procedures:
1. Take the MaxSortin® L-Separation Column (MS-CL01) and place it on the MACS sorter (130-090-976), and then rinse it twice with 1 mL of MaxSortin® cell sorting buffer.
2. Add the sample, which has been incubated with MaxSortin® series of cell sorting magnetic beads and cells, into the L-Separation Column. After it flows out naturally, add the cell sorting buffer in two portions, 3 mL each time. Collect the effluent in a 15 mL tube. The collected cells are the negatively selected cells.
3. After all the cell sorting buffer has flowed out, remove the L-Separation Column from the MACS sorter and place it in another new 15 mL centrifuge tube (mark it properly). Add 3 mL of cell sorting buffer into the L-Separation Column and use a plunger to push the liquid out directly. The collected cells are the positively selected cells.
4. Count the cells and conduct flow cytometry detection.
This product is only applicable to in vitro cell culture and cannot be directly used for clinical treatment.
