Onye mmeri mmezi na gburugburu oke okpomọkụ! T&L Biotechnology ewepụtala Protein bFGF Thermostable Ọhụrụ
Basic Fibroblast Growth Factor (bFGF), onye so na ezinụlọ fibroblast growth factor, na-ebute ụzọ ntụgharị akara site na ijikọ na ịgbalite ndị na-anabata ya na targe.T Cell elu. bFGF na-achịkwa mmụba nke ụdị sel dị iche iche, gụnyere mkpụrụ ndụ endothelial, neurons, chondrocytes, mkpụrụ ndụ akwara dị nro, melanocytes, na adipocytes. Na mgbakwunye, bFGF na-etinye aka na usoro ndu dị ka mmeghari ohuru nke cell, ihe dị iche iche, mmepe, nrụzi anụ ahụ, na mmụgharị. Dị ka ihe dị mkpa maka uto cell, a na-eji bFGF eme ihe na omenala in vitro nke ụdị cell dị iche iche.
Nnyocha na-egosi na bFGF anaghị eguzosi ike n'ihe n'okpuru ọnọdụ physiological, yana ọkara ndụ ihe dịka awa 8 na omenala cell mammalian. Nkwụsi ike dị ala na ọkara ndụ nke bFGF na-eduga n'ihe ịma aka na omenala cell, na-achọ nkwado ihe na-eto eto ugboro ugboro ma ọ bụ ngbanwe nke ọkara kwa ụbọchị. Protein bFGF na-ekpo ọkụ, nke emepụtara site na mgbanwe nke bFGF eke, na-edozi ihe ịma aka a. Ọ na-ejigide ike ọrụ ndu nke bFGF eke ka ọ na-enweta nkwụsi ike ọkụ, na-ejigide iguzosi ike n'ezi ihe nhazi yana ọrụ protein n'ofe oke okpomọkụ.
Embrayo mmadụ Cell Stems (hESCs) na sel sel stem pluripotent (iPSC) nwere ike ịwapụta n'ụdị sel somatic niile. N'oge in vitro omenala hESCs na iPSC, bFGF dị mkpa maka idowe spripot cell cell. Nnyocha na-egosi na ndị hESC mebere ya na bFGF na-ekpo ọkụ na-etolite ógbè na-enweghị atụ ngwa ngwa (Fig 1A) ma gosipụta mmụba mmụba n'oge omenala ogologo oge (Fig 1B). Nnyocha ndị ọzọ na-ekpughe na bFGF na-ekpo ọkụ na-akwalite mmụba hESC ka ọ na-akwado nkwupụta nke akara pluripotency (dịka, OCT4, SOX2, KLF4), na-akwado ikike ya ichekwa hESC pluripotency-ihe dị oke mkpa maka ngwa omenala cell.
A: Ọkwa ntolite nke hESC n'ụbọchị 2 n'okpuru ọnọdụ omenala enweghị nri nwere ụdị bFGF dị iche iche.
B: Tụnyere ọnụego uto cell n'oge ọdịbendị na-aga n'ihu na WT-bFGF (ụdị anụ ọhịa) ma ọ bụ TS-bFGF (thermostable) n'okpuru ọnọdụ enweghị nri.
Mwepụta ngwaahịa
Protein bFGF mmadụ recombinant (Heat Stable)
Njirimara ngwaahịa
- Thermostable
- Ịdị ọcha dị elu (>90%)
- Omume dị elu
- Endotoxin dị ala (
- Ihe na-adịghị mma
- Mycoplasma-free
Arụmọrụ ngwaahịa
Ngwa Scenario
Mgbe agbakwunyere na iPSC omenala, enwere ike ịme ngbanwe ọkara kwa ụbọchị 2 ọ bụla.
Dị ka e gosiri na fig. 5, T&L's Recombinant Human bFGF Protein (GMP-TL901) na Thermostable bFGF ka etinyere na AMMS® Pluripotent Stem Cell Medium (AS-35) maka omenala iPSC. Na ọkara agbakwunyere na bFGF thermostable, mgbanwe mgbanwe kwa ụbọchị ma ọ bụ ụbọchị ọ bụla na-eme ka ọnụọgụ na nha ógbè ka mma nke ukwuu ma e jiri ya tụnyere bFGF oge niile. N'ụzọ doro anya, ịdị ndụ cell egosighi nnukwu ọdịiche dị n'etiti ụkpụrụ kwa ụbọchị na nke ụbọchị ọ bụla nwere bFGF na-ekpo ọkụ, na-akwado nkwado ya maka omenala iPSC na-akwụ ụgwọ nke ọma.
Ị nwere mmasị ịnwale Protein bFGF Thermostable New-Generation?
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T&L Biotechnology Co., Ltd. na-elekwasị anya na nyocha na mmepe nke elu, akụrụngwa GMP-ọkwa na reagents maka cell na Usoro ọgwụgwọ Gene (CGT). Anyị na-agba mbọ na-enye ngụkọta ngwọta maka CGT ahịa site anyị keukwu ngwaahịa na ọrụ àjà. Ngwaahịa anyị gụnyere cell nkewa na mgbanaka magnetik beads, Eukaryotic/prokaryotic recombinant proteins, ihe na-enweghị serum, ngwa omenala cell, na ndị ọzọ.
Anyị na-arụ ọrụ nyocha na ụlọ nyocha mmepe yana ogbako dị ọcha nke ọkwa GMP 3,200-square. Akụrụngwa ndị a gụnyere ikpo okwu maka mmepe magnetic bead nkewa nkewa, eukaryotic na prokaryotic protein expression engineering, na mmepe ọkara na-enweghị serum. Anyị na-agbaso ụkpụrụ ISO 13485 na ISO 9001 Quality Management System. Na mgbakwunye, ụfọdụ ngwaahịa anyị nwere ntinye akwụkwọ FDA DMF.