| Aha | Nọmba edemede | Nkọwapụta | Ọnọdụ Nchekwa | Ndụ shelf |
| MaxSortin® CD4 Nkewa Magnetic Beads | TL-623 | 2 ml maka 1 × 10 ⁹ sel mkpokọta | 2-8 Celsius C | Ọnwa isii |
| Ihe nchekwa ihe nhazi cell MaxSortin® | MS-BF100 | 100 ml | 2-8 Celsius C | Ọnwa iri na abụọ |
| Kọlụm nkewa MaxSortin® L | MS-CL01 | 1 ibe | 10-35 Celsius C | Ọnwa iri na abụọ |
Ụdị na-emeghachi omume: mmadu
Endotoxin magnetik bead:
Ọdịdị Igwe Igwe Igwe Ọdụdọ: mmiri mmiri agba aja aja
Enwere ike iji Kit nkewa MaxSortin® CD4 dozie sel CD4+ T mmadụ. Site na incubating nanoscale CD4 nkewa magnetik beads nwere mkpụrụ ndụ, enwere ike nweta nhazi nke sel CD4+ T. The nanoscale CD4 nkewa magnetik beads na-incubated na mpụta ọbara mononuclear sel (PBMCs) na mgbe ahụ doro magnetik nkewa, nke na-enyere ike nkewa na enrichment nke CD4+ T cell, na-ekere òkè na-eme ka CD4+ T cell dị ọcha na ọdabara na mmepụta nke cell ọgwụgwọ ngwaahịa.
1. Weghachite PBMC mmadụ na nchekwa PBS nwere 1% serum albumin (HSA). Were nlele maka ịgụta ma bufee sel 1 × 10⁷ n'ime tube EP 1.5 ml. Mgbe ahụ centrifuge na 1500 rpm maka 5 nkeji.
2. Tụfuo ihe dị elu, were 80 μL nke MaxSortin® Cell Sorting Buffer ka ọ kwụsịtụ sel, tinye 20 μL nke CD4 nkewa magnetik beads, gwakọta nke ọma wee tinye ha na friji na 2 - 8 Celsius C maka incubation maka nkeji 15.
3. Were MaxSortin® L-Separation Column ma tinye ya na ụdị ụdị cell magnetik-activated (MACS), wee kpochaa ya ugboro abụọ na 1 ml nke MaxSortin® Cell Sorting Buffer.
4. Were ihe nlele nke mechachara na refrjiraeto na 2 - 8 Celsius C, tinye 1 mL nke ngwọta MACS, centrifuge na 1500 rpm maka nkeji 5, ma tụfuo ihe dị elu.
5. Tinye 1 ml nke MaxSortin® Cell Sorting Buffer ka iweghachite mkpụrụ ndụ, tinye ihe nlele n'ime kọlụm nkewa. Mgbe ọ gachara, tinye MaxSortin® Cell Sorting Buffer n'akụkụ abụọ, 1 ml oge ọ bụla. Chịkọta ihe na-esi ísì ụtọ na tube centrifuge 15 ml.
6. Mgbe MaxSortin® Cell Sorting Buffer ga-apụ, wepụ kọlụm nkewa na ụdị MACS ma tinye ya na tube centrifuge 15 mL ọhụrụ ọzọ. Tinye 3 ml nke MaxSortin® Cell Sorting Buffer n'ime kọlụm nkewa wee jiri piston na-abịa na kọlụm nkewa wee kwapụ mmiri mmiri ozugbo.
7. Debe tube centrifuge 15 ml nke nwere mmiri mmiri anakọtara n'ime centrifuge kwụ ọtọ na centrifuge na 1500 rpm maka nkeji 5.
8. Mgbe centrifugation, wụsa supernatant, were 1 mL nke 1 × Dulbecco's phosphate-buffered saline (DPBS) ngwọta resupend cell, gụọ cell na-eduzi eruba nchọpụta cytometry.
Mgbe ị na-ejikọta bead magnetik na mkpụrụ ndụ, ọ dị mkpa ijikọta ha nke ọma iji melite arụmọrụ nhazi.
