| Lub npe | Tshooj Naj Npawb | Specification | Kev cia khoom | Txee lub neej |
| MaxSortin® CD4 Separation Sib Nqus Beads | TSI-623 | 2 mL rau 1 × 10⁹ tag nrho cov hlwb | 2-8 ° C | 6 hli |
| MaxSortin® Cell Sorting Buffer | MS-BF100 | 100 mL | 2-8 ° C | 12 hli |
| MaxSortin® L-Separation Kem | Qauv TSIS MUAJ: MS-CL01 | 1 qob | 10-35 ° C | 12 hli |
Reactive Hom: Neeg
Sib nqus Bead Endotoxin:
Magnetic Bead Appearance: Xim av kua
MaxSortin® CD4 Separation Kit tuaj yeem siv los txheeb xyuas tib neeg CD4+ T hlwb. Los ntawm incubating nanoscale CD4 sib cais sib nqus hlaws nrog cov hlwb, kev txheeb xyuas ntawm CD4 + T hlwb tuaj yeem ua tiav. Cov nanoscale CD4 sib cais magnetic hlaws yog incubated nrog peripheral ntshav mononuclear hlwb (PBMCs) thiab ces raug sib nqus sib cais, uas ua rau kev sib cais thiab enrichment ntawm CD4 + T hlwb, ua si lub luag hauj lwm nyob rau hauv purifying CD4 + T hlwb thiab muaj feem xyuam rau zus tau tej cov cell kho cov khoom.
1. Rov ua dua tib neeg PBMCs hauv PBS tsis muaj 1% human serum albumin (HSA). Siv ib qho piv txwv rau suav thiab hloov 1 × 10⁷ hlwb rau hauv 1.5 mL EP raj. Tom qab ntawd centrifuge ntawm 1500 rpm rau 5 feeb.
2. Tshem tawm cov supernatant, noj 80 μL ntawm MaxSortin® Cell Sorting Buffer kom rov ua cov hlwb, ntxiv 20 μL ntawm CD4 sib cais sib nqus hlaws, sib tov zoo thiab muab tso rau hauv lub tub yees ntawm 2 - 8 ° C rau incubation rau 15 feeb.
3. Siv MaxSortin® L-Separation Column thiab muab tso rau ntawm lub tshuab magnetic-activated cell sorting (MACS) sorter, thiab yaug nws ob zaug nrog 1 mL ntawm MaxSortin® Cell Sorting Buffer.
4. Nqa cov qauv uas tau ua tiav incubation tawm ntawm lub tub yees ntawm 2 - 8 ° C, ntxiv 1 mL ntawm MACS tov, centrifuge ntawm 1500 rpm rau 5 feeb, thiab muab pov tseg lub supernatant.
5. Ntxiv 1 mL ntawm MaxSortin® Cell Sorting Buffer rau resuspend lub hlwb, ntxiv cov qauv rau hauv kab sib cais. Tom qab nws ntws tawm lawm, ntxiv MaxSortin® Cell Sorting Buffer hauv ob feem, 1 mL txhua zaus. Sau cov effluent hauv 15 mL centrifuge raj.
6. Tom qab tag nrho cov MaxSortin® Cell Sorting Buffer tau ntws tawm, tshem cov kab sib cais los ntawm MACS sorter thiab muab tso rau hauv lwm lub raj 15 mL centrifuge tshiab. Ntxiv 3 mL ntawm MaxSortin® Cell Sorting Buffer rau hauv kem kev sib cais thiab siv lub piston uas tuaj nrog cov kab sib cais kom thawb cov kua tawm ncaj qha.
7. Muab lub raj 15 mL centrifuge uas muaj cov kua sau ua ke rau hauv kab rov tav centrifuge thiab centrifuge ntawm 1500 rpm rau 5 feeb.
8. Tom qab centrifugation, ncuav tawm cov supernatant, noj 1 mL ntawm 1 × Dulbecco's phosphate-buffered saline (DPBS) daws kom rov ua kom cov hlwb, suav cov hlwb thiab ua cov cytometry ntws.
Thaum incubating cov hlaws sib nqus nrog cov hlwb, nws yog ib qho tsim nyog yuav tsum sib tov kom huv si txhawm rau txhim kho kev sib cais.
