Ka Poʻokela Hoʻoponopono i nā Kaiapuni Kūlana Kiʻekiʻe! Hoʻokuʻu ʻia ʻo T&L Biotechnology i ka mea hou-Generation Thermotable bFGF Protein
ʻO ka Basic Fibroblast Growth Factor (bFGF), he lālā o ka ʻohana fibroblast growth factor, hoʻāla i nā ala transduction hōʻailona ma o ka hoʻopaʻa ʻana a me ka hoʻōla ʻana i nā mea loaʻa ma ka pae.T Cell nā ʻili. Hoʻoponopono ka bFGF i ka hoʻonui ʻana o nā ʻano cell like ʻole, e komo pū me nā cell endothelial, neurons, chondrocytes, nā ʻiʻo ʻiʻo maʻemaʻe, melanocytes, a me nā adipocytes. Hoʻohui ʻia, pili ka bFGF i nā kaʻina olaola e like me ka cell self-renewal, differentiation, development, tissue repair, and regeneration. Ma ke ʻano he kumu koʻikoʻi no ka ulu ʻana o ke kelepona, hoʻohana nui ʻia ka bFGF i ka moʻomeheu in vitro o nā ʻano cell like ʻole.
Hōʻike nā haʻawina ʻaʻole paʻa ka bFGF ma lalo o nā kūlana physiological, me ka hapalua o ke ola ma kahi o 8 mau hola i nā moʻomeheu cell mammalian. ʻO ka haʻahaʻa haʻahaʻa haʻahaʻa haʻahaʻa a me ka hapalua o ke ola o ka bFGF e alakaʻi i nā pilikia i ka moʻomeheu cell, e koi ana i ka hoʻonui pinepine ʻana i ka mea ulu a i ʻole ka hoʻololi ʻana i kēlā me kēia lā. ʻO ka protein bFGF thermostable, i hana ʻia ma o ka hoʻololi ʻana o ka bFGF maoli, e hoʻoponopono i kēia pilikia. Mālama ʻo ia i ka hana olaola ikaika o ka bFGF maoli i ka wā e loaʻa ai ka hoʻonui ʻia ʻana o ke kūpaʻa wela, ka mālama pono ʻana i ka hana a me ka hana protein ma waena o kahi ākea ākea.
Embryonic kanaka Pūnaehana Puʻupuʻus (hESCs) a me ka pluripotent stem cell (iPSCs) hiki ke hoʻokaʻawale i nā ʻano cell somatic āpau. I ka moʻomeheu in vitro o nā hESC a me nā iPSC, pono ka bFGF no ka mālama ʻana i ka pluripotency stem cell. Hōʻike ka noiʻi ʻana i nā hESC i hoʻoulu ʻia me ka bFGF thermostable e hoʻokumu i nā kolone i hoʻokaʻawale ʻole ʻia (Fig. 1A) a hōʻike i nā helu hoʻonui i hoʻonui ʻia i ka moʻomeheu lōʻihi (Fig. 1B). Hōʻike nā haʻawina hou aʻe e hāpai ana ka thermostable bFGF i ka hoʻonui ʻana i ka hESC i ka wā e kākoʻo ana i ka hōʻike ʻana o nā māka pluripotency (eg, OCT4, SOX2, KLF4), e hōʻoia ana i kona hiki ke mālama i ka hESC pluripotency - kahi hiʻohiʻona koʻikoʻi no nā noi moʻomeheu cell.
A: Ke kūlana ulu o nā hESC ma ka lā 2 ma lalo o nā kūlana moʻomeheu hānai ʻole me nā ʻano like ʻole bFGF.
B: Ka hoʻohālikelike ʻana i ka nui o ka ulu ʻana o ke kelepona ma ka moʻomeheu hoʻomau me WT-bFGF (wild-type) a i ʻole TS-bFGF (thermotable) ma lalo o nā kūlana hānai ʻole.
Hoʻolaha Huahana
Paʻa Paʻa bFGF kanaka hou (Heat Stable)
Nā hiʻohiʻona huahana
- Māmā
- Maʻemaʻe Kiʻekiʻe (>90%)
- Hana Kiʻekiʻe
- Endotoxin Haʻahaʻa (
- Paʻa ʻole
- ʻAʻole Mycoplasma
Hana Huahana
pplication Scenario
Ke hoʻohui ʻia i ke ʻano moʻomeheu iPSC, hiki ke hana ʻia ke pani ʻana i kēlā me kēia 2 lā.
E like me ka mea i hōʻike ʻia ma ka Fig. 5, ua hoʻohana ʻia ka T&L's Recombinant Human bFGF Protein (GMP-TL901) a me Thermotable bFGF i AMMS® Pluripotent Stem Cell Medium (AS-35) no ka moʻomeheu iPSC. Ma ka waena i hoʻohui ʻia me ka thermostable bFGF, ʻoi aku ka maikaʻi o ka hoʻololi ʻana i kēlā me kēia lā a i ʻole kēlā me kēia lā i nā helu koloni a me nā nui i hoʻohālikelike ʻia me ka bFGF maʻamau. ʻO ka mea nui, ʻaʻole i hōʻike ʻia ka ʻokoʻa koʻikoʻi ma waena o nā protocols o kēlā me kēia lā a me kēlā me kēia lā me ka thermostable bFGF, e hōʻoia ana i kona kūpono no ka moʻomeheu iPSC kūpono.
Makemake ʻoe e hoʻāʻo i kā mākou New-Generation Thermotable bFGF Protein?
E hoʻokaʻaʻike mai iā mākou i kēia lā e noi i kahi laʻana manuahi a ʻike mua i nā pōmaikaʻi no kāu noiʻi!
Ke kālele nei ʻo T&L Biotechnology Co., Ltd. i ka noiʻi ʻana a me ka hoʻomohala ʻana o ka upstream, GMP-grade maka a me nā reagents no ke kelepona a me Hoʻoponopono Gene (CGT). Kūpaʻa mākou i ka hāʻawi ʻana i nā hāʻina holoʻokoʻa no nā mea kūʻai aku CGT ma o kā mākou huahana a me nā lawelawe lawelawe. Loaʻa i kā mākou huahana kaʻawale ʻana o ke kelepona a activation magnetic beads, eukaryotic/prokaryotic recombinant proteins, serum-free medium, cell culture kits, a oi aku.
Hana mākou i kahi keʻena noiʻi a me ka hoʻomohala ʻana a me kahi hale hana maʻemaʻe 3,200-square-mika pae GMP. Loaʻa kēia mau mea i nā paepae no ka hoʻomohala ʻana i ka bead magnetic separation cell, eukaryotic a me prokaryotic protein expression engineering, a me ka hoʻomohala ʻana i ka serum-free medium development. Hoʻopili mākou i ka ISO 13485 a me ISO 9001 Quality Management System Standards. Eia kekahi, aia kekahi o kā mākou huahana i nā faila DMF FDA.