| inoa | Helu ʻatikala | Hōʻike | Nā Kūlana Waihona | Ola Pahu |
| MaxSortin® CD4 Hoʻokaʻawale Mākēneki Lei | TL-623 | 2 mL no 1×10⁹ ka huina pau | 2 - 8 °C | 6 mahina |
| ʻO MaxSortin® Pūnaehana Hoʻokaʻawale Pūnaewele | MS-BF100 | 100 mL | 2 - 8 °C | 12 mahina |
| MaxSortin® L-Kāuā Hoʻokaʻawale | MS-CL01 | 1 apana | 10 - 35 °C | 12 mahina |
Nā ʻano ʻano hoʻihoʻi: Kanaka
ʻO Endotoxin ʻo Magnetic Bead:
ʻIke ʻia ʻo Magnetic Bead: Wai ʻulaʻula
Hiki ke hoʻohana ʻia ka MaxSortin® CD4 Separation Kit e hoʻokaʻawale i nā pūnaewele CD4+ T kanaka. Ma o ka incubating nanoscale CD4 separation magnetic beads me nā pūnaewele, hiki ke hoʻokō ʻia ka hoʻokaʻawale ʻana o nā cell CD4+ T. Hoʻokomo ʻia ka nanoscale CD4 separation magnetic beads me ka peripheral blood mononuclear cell (PBMCs) a laila e hoʻokaʻawale ʻia ka hoʻokaʻawale ʻana, e hiki ai i ka hoʻokaʻawale ʻana a me ka hoʻonui ʻana i nā cell CD4 + T, e pāʻani ana i ka hoʻomaʻemaʻe ʻana i nā cell CD4 + T a pili i ka hana ʻana o nā huahana cell therapy.
1. Hoʻopau hou i nā PBMC kanaka i loko o ka pahu PBS i loaʻa ka 1% albumin serum kanaka (HSA). E lawe i kahi laʻana no ka helu ʻana a hoʻololi i nā ʻāpana 1x10⁷ i loko o kahi paipu EP 1.5 mL. A laila centrifuge ma 1500 rpm no 5 mau minuke.
2. E hoʻolei i ka supernatant, e lawe i 80 μL o MaxSortin® Cell Sorting Buffer e hoʻomaha hou i nā keena, e hoʻohui i 20 μL o CD4 separation magnetic beads, e hui maikaʻi a laila e waiho iā lākou i loko o ka friji ma 2 - 8 ° C no ka incubation no 15 mau minuke.
3. E lawe i ke Column MaxSortin® L-Separation Column a kau ia ma luna o ka mea hoʻoheheʻe ʻana i ke kelepona hoʻohana magnetic (MACS), a holoi ʻelua me ka 1 mL o MaxSortin® Cell Sorting Buffer.
4. E lawe i ka hāpana i hoʻopau i ka incubation mai loko mai o ka friji ma 2 - 8 °C, e hoʻohui i 1 mL o ka solution MACS, centrifuge ma 1500 rpm no 5 mau minuke, a hoʻolei i ka supernatant.
5. E hoʻohui i 1 mL o MaxSortin® Cell Sorting Buffer e hoʻomaha hou i nā pūnaewele, e hoʻohui i ka hāpana i loko o ke kolamu kaʻawale. Ma hope o ka kahe maoli ʻana, hoʻohui i ka MaxSortin® Cell Sorting Buffer i ʻelua ʻāpana, 1 mL i kēlā me kēia manawa. E hōʻiliʻili i ka effluent i loko o kahi paipu centrifuge 15 mL.
6. Ma hope o ka kahe ʻana o ka MaxSortin® Cell Sorting Buffer, e wehe i ke kolamu hoʻokaʻawale mai ka MACS sorter a waiho i loko o kahi paipu centrifuge 15 mL hou. E hoʻohui i 3 mL o MaxSortin® Cell Sorting Buffer i loko o ke kolamu hoʻokaʻawale a hoʻohana i ka piston e hele mai me ke kolamu hoʻokaʻawale e pana pololei i ka wai.
7. E kau i ka paipu centrifuge 15 mL i loko o ka wai i ohiia i loko o ka centrifuge a me ka centrifuge ma 1500 rpm no 5 minuke.
8. Ma hope o ka centrifugation, ninini i ka supernatant, e lawe i 1 mL o 1× Dulbecco's phosphate-buffered saline (DPBS) solution no ka hoʻomaha hou ʻana i nā keena, helu i nā keena a hana i ka ʻike cytometry kahe.
I ka hoʻoulu ʻana i nā peʻa magnetic me nā cell, pono e hoʻohui pono iā lākou i mea e hoʻomaikaʻi ai i ka hana ʻana.
