Gwarzon Gyara a cikin Mahalli masu zafi! T&L Biotechnology A hukumance Ya ƙaddamar da Sabbin Ƙarfafa Ƙwararrun Ƙwararru na bFGF Protein
Babban Factor Growth Factor (bFGF), memba na dangin fibroblast girma factor iyali, yana haifar da siginar hanyoyin canzawa ta hanyar ɗaure zuwa da kunna masu karɓa akan manufa.T Cell saman. bFGF yana daidaita yaduwar nau'ikan sel daban-daban, gami da ƙwayoyin endothelial, neurons, chondrocytes, ƙwayoyin tsoka mai santsi, melanocytes, da adipocytes. Bugu da ƙari, bFGF yana shiga cikin hanyoyin nazarin halittu kamar sabuntar tantanin halitta, rarrabuwa, haɓakawa, gyaran nama, da sabuntawa. A matsayin muhimmin abu don haɓakar tantanin halitta, ana amfani da bFGF sosai a cikin al'adun in vitro na nau'ikan tantanin halitta daban-daban.
Nazarin ya nuna cewa bFGF ba shi da kwanciyar hankali a ƙarƙashin yanayin ilimin lissafi, tare da rabin rayuwa na kusan sa'o'i 8 a cikin al'adun sel masu shayarwa. Ƙananan kwanciyar hankali na thermal da gajeriyar rabin rayuwar bFGF suna haifar da ƙalubale a al'adar tantanin halitta, suna buƙatar ƙarin abubuwan haɓaka haɓaka ko sauyawa na yau da kullun. Protein bFGF mai zafi, wanda aka ƙera ta hanyar gyara bFGF na halitta, yana magance wannan ƙalubale. Yana riƙe ƙwaƙƙarfan ayyukan ilimin halitta na bFGF na halitta yayin samun ingantacciyar kwanciyar hankali mai zafi, kiyaye amincin tsari da ayyukan furotin a cikin kewayon zafin jiki mai faɗi.
Dan Adam amfrayo Kwayoyin Tsawos (hESCs) da ƙwanƙwarar ƙwayoyin cuta masu ƙarfi (iPSCs) suna da yuwuwar bambanta zuwa kowane nau'in tantanin halitta. A yayin al'adun in vitro na hESCs da iPSCs, bFGF yana da mahimmanci don kiyaye yawan ƙarfin sel. Bincike ya nuna cewa hESCs al'ada tare da thermostable bFGF suna samar da yankuna marasa bambanci da sauri (Fig. 1A) kuma suna nuna haɓakar haɓakawa yayin al'ada na dogon lokaci (Fig. 1B). Ƙarin karatu ya nuna cewa bFGF mai zafi yana inganta haɓakar hESC yayin da yake ci gaba da bayyana alamun da yawa (misali, OCT4, SOX2, KLF4), yana tabbatar da ikonsa na adana hESC pluripotency-mahimmin fasali don aikace-aikacen al'adun tantanin halitta.
A: Matsayin girma na hESCs a ranar 2 a ƙarƙashin yanayin al'ada mara amfani tare da bambance-bambancen bFGF.
B: Kwatanta ƙimar haɓakar tantanin halitta yayin ci gaba da al'ada tare da WT-bFGF (nau'in daji) ko TS-bFGF (mai zafi mai zafi) ƙarƙashin yanayi mara amfani.
Kaddamar da samfur
Recombinant Human bFGF Protein (Heat Stable)
Siffofin Samfur
- Thermostable
- Babban Tsafta (> 90%)
- Babban Ayyuka
- Low Endotoxin (
- Bakara
- Mycoplasma-Free
Ayyukan Samfur
aikace-aikace Scenario
Lokacin da aka ƙara zuwa matsakaicin al'adun iPSC, ana iya yin maye gurbin matsakaici kowane kwana 2.
Kamar yadda aka nuna a cikin siffa 5, T&L's Recombinant Human bFGF Protein (GMP-TL901) da Thermostable bFGF an yi amfani da AMMS® Pluripotent Stem Cell Medium (AS-35) don al'adun iPSC. A cikin matsakaici wanda aka ƙara tare da bFGF mai zafi, matsakaita na yau da kullun ko kowace-rana na canje-canje sun inganta lambobi da girma na mazauna sosai idan aka kwatanta da bFGF na yau da kullun. Musamman ma, yuwuwar tantanin halitta bai nuna wani gagarumin bambanci tsakanin ka'idojin yau da kullun da na yau da kullun tare da bFGF mai zafi ba, yana tabbatar da dacewarta ga al'adun iPSC mai inganci.
Kuna sha'awar gwada Sabon-ƙarni Mai Ƙarfafa Ƙwararrun Protein bFGF?
Tuntube mu a yau don neman samfurin kyauta kuma ku dandana fa'idodin da hannu don bincikenku!
T&L Biotechnology Co., Ltd. yana mai da hankali kan bincike da haɓaka haɓakawa, albarkatun albarkatun GMP da reagents don tantanin halitta da Gene Therapy (CGT). Mun himmatu don samar da jimillar mafita ga abokan cinikin CGT ta hanyar samfuran samfuranmu da sadaukarwar sabis. Kayayyakin mu sun haɗa da rabuwar tantanin halitta kuma kunna Magnetic beads, eukaryotic/prokaryotic sunadaran sake haxawa, matsakaici mara amfani, kayan al'adun tantanin halitta, da sauransu.
Muna aiki da dakin gwaje-gwaje na bincike da haɓakawa da tsaftataccen matakin GMP mai girman murabba'in mita 3,200. Waɗannan wurare sun haɗa da dandamali don haɓakar haɓakar ƙwanƙwasa maganadisu, eukaryotic da injiniyan furotin prokaryotic, da ci gaban matsakaici mara amfani. Muna bin ka'idodin ISO 13485 da ISO 9001 Ingancin Tsarin Gudanar da Ingancin. Bugu da ƙari, wasu samfuranmu suna da takaddun FDA DMF.