| Suna | Lamba Labari | Ƙayyadaddun bayanai | Yanayin Ajiya | Rayuwar Rayuwa |
| MaxSortin® CD4 Rarraba Magnetic Beads | Farashin TL-623 | 2 ml na 1 × 10⁹ jimlar sel | 2-8 ° C | Wata 6 |
| MaxSortin® Salon Rarraba Buffer | MS-BF100 | 100 ml | 2-8 ° C | watanni 12 |
| MaxSortin® L-Shafin Rabuwa | MS-CL01 | guda 1 | 10-35 ° C | watanni 12 |
Nau'o'in Reactive: Mutum
Magnetic Bead Endotoxin:
Bayyanar Bead Magnetic: Ruwan ruwa
Ana iya amfani da Kit ɗin Rabewar MaxSortin® CD4 don ware ƙwayoyin CD4+ T na ɗan adam. Ta hanyar haɗa nanoscale CD4 separation magnetic beads tare da sel, ana iya samun rarrabuwar ƙwayoyin CD4+ T. The nanoscale CD4 separation Magnetic beads ana cuba tare da na gefe jini mononuclear Kwayoyin (PBMCs) sa'an nan kuma hõre da Magnetic rabuwa, wanda damar da rabuwa da wadãtar da CD4+ T Kwayoyin, taka rawa a tsarkake CD4+ T Kwayoyin kuma ya shafi samar da cell far kayayyakin.
1. Dakatar da PBMCs na ɗan adam a cikin buffer PBS mai ɗauke da 1% albinin ɗan adam (HSA). Ɗauki samfurin don kirgawa kuma canja wurin sel 1 × 10⁷ zuwa cikin bututun EP 1.5 ml. Sa'an nan centrifuge a 1500 rpm na minti 5.
2. Yi watsi da maɗaukaki, ɗauki 80 μL na MaxSortin® Cell Sorting Buffer don sake dakatar da sel, ƙara 20 μL na CD4 separation magnetic beads, haɗuwa da kyau sannan a sanya su a cikin firiji a 2 - 8 ° C don shiryawa na mintina 15.
3. Ɗauki MaxSortin® L-Separation Column kuma sanya shi a kan nau'in magnetic-activated cell sorting (MACS), sa'annan ku wanke shi sau biyu tare da 1 ml na MaxSortin® Cell Sorting Buffer.
4. Ɗauki samfurin da ya gama shiryawa daga cikin firiji a 2 - 8 ° C, ƙara 1 ml na maganin MACS, centrifuge a 1500 rpm na minti 5, kuma jefar da supernatant.
5. Ƙara 1 ml na MaxSortin® Cell Sorting Buffer don sake dakatar da sel, ƙara samfurin a cikin ginshiƙin rabuwa. Bayan ya fita ta dabi'a, ƙara MaxSortin® Cell Sorting Buffer a cikin kashi biyu, 1 ml kowane lokaci. Tattara abubuwan da suka zubar a cikin bututun sintifuge na 15 ml.
6. Bayan duk MaxSortin® Cell Sorting Buffer ya fita, cire ginshiƙin rabuwa daga mai rarraba MACS kuma sanya shi a cikin wani sabon 15 mL centrifuge tube. Ƙara 3 ml na MaxSortin® Cell Sorting Buffer a cikin ginshiƙin rabuwa kuma yi amfani da piston wanda ya zo tare da ginshiƙin rabuwa don tura ruwa kai tsaye.
7. Sanya bututun centrifuge na 15 ml wanda ke dauke da ruwan da aka tattara a cikin centrifuge na kwance da centrifuge a 1500 rpm na mintuna 5.
8. Bayan centrifugation, zuba fitar da supernatant, dauki 1 mL na 1 × Dulbecco ta phosphate-buffered Saline (DPBS) bayani don sake dakatar da sel, ƙidaya sel da kuma gudanar da kwarara cytometry gano.
Lokacin shigar da beads na maganadisu tare da sel, ya zama dole a haɗa su sosai don haɓaka yadda ya dace.
