ActCel T Cell Activation Reagent

ActCel T cell activation reagent is synthesized by coupling a nanomatrix with anti-human CD3 antibody and anti-human CD28 antibody, and it can gently and efficiently activate T cells or peripheral blood mononuclear cells (PBMCs).

1.1 Conduct the experiment at a titer of 1:200. The specific amounts to be added are shown in the following table:

1.2 Taking 1×10⁶ T cells as an example: Add 995 μL of X-VIVOTM 15 medium and 5 μL of T cell activation reagent (containing 200 IU/mL interleukin-2 (IL-2)) to 1×10⁶ T cells. Then culture them at 37 °C under 5% CO₂ for 2 days.
1.3 After culturing for 2 - 3 days, centrifuge at 300g for 10 minutes to remove the supernatant. Then add 2 mL of fresh complete medium to remove the T cell activation reagent and continue culturing at 37 °C under 5% CO₂.
1.4 Every 2 days, pipette the cell suspension thoroughly to mix evenly. Add twice the volume of fresh complete medium (the ratio of the original volume to the supplementary volume is 1:2), count the cells and calculate the amplification factor. Then culture them in a 37 °C, 5% CO₂ incubator. For example, if the medium is supplemented on day 4 and the original volume is 2 mL, 4 mL of medium should be added, and the final total volume will be 6 mL.

This product is only applicable to in vitro cell culture and cannot be directly used for clinical treatment.