Recombinant Human VEGF165 Protein

Stock: In Stock
Model: GMP-TL612

Description

Data Figures

Characterization

Document Files

Product Overview

Recombinant Human Transforming Growth Factor Beta 3 (TGF-β3) is a multifunctional cytokine involved in the regulation of cell growth, differentiation, and immune responses. By binding to its receptors, TGF-β3 activates downstream signaling pathways that regulate extracellular matrix production and tissue repair, making it an essential tool in cellular biology research.

Key Features

1.High Purity: Purified using advanced chromatographic techniques, this product achieves a purity level of over 95%, ensuring accurate and reproducible experimental results.

2.High Biological Activity: Confirmed through multiple activity assays, this VEGF165 protein effectively induces endothelial cell proliferation and migration, making it suitable for a range of research applications.

3.Batch Consistency: Produced under stringent quality control measures to ensure high consistency between batches, reducing variability and enhancing experimental reliability.

4.Animal-Free: Manufactured without the use of animal-derived components, adhering to animal-free standards and broadening its applicability for diverse research needs.

Applications

• Oncology: Study the role of VEGF165 in tumor angiogenesis, providing insights into cancer progression and the development of anti-angiogenic therapies.

• Cardiovascular Research: Investigate the effects of VEGF165 on vascular health and its potential in treating cardiovascular diseases, including ischemic conditions.

• Tissue Engineering: Utilize VEGF165 in the development of engineered tissues and organs to promote vascularization and tissue viability.

• Wound Healing: Explore the role of VEGF165 in wound healing processes, including tissue repair and regeneration, offering potential therapeutic approaches.

Specifications and Packaging

• Specifications: Available in various sizes to meet different experimental requirements, including 10μg, 50μg, 100μg, and 1mg.

• Packaging: Supplied in sterile lyophilized form to ensure stability and facilitate convenient storage and transportation.

Storage and Stability

• Storage: Store at -20°C or below to maintain product stability and biological activity. Avoid repeated freeze-thaw cycles to prevent degradation.

• Stability: Stable for long-term storage under recommended conditions, ensuring reliable performance in research applications.

Instructions for Use

1.Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.

2.Prepare and dilute the solution according to experimental requirements to achieve accurate and reproducible results.

3.Avoid mixing with other similar products to prevent cross-reactivity and potential interference.

Cautions

• This product is intended for research use only and is not suitable for clinical diagnostics or therapeutic applications.

• Handle the product according to provided instructions to maintain protein activity and integrity.

• For any issues or inquiries regarding the product, please contact our technical support team.

Cytokines
Expression Host	HEK293 Cells
Synonym	MVCD1,VEGF,VEGF165,VPF
Protein Sequence	A DNA sequence encoding the human VEGF165 (NP_001165097.1) was expressed with a polyhistidine tag at the C-terminus.
Molecular Mass	The Recombinant Human VEGF165 consists of 193 amino acids and predicts a molecular mass of 22.3 kDa.
QC Testing Purity	> 90 % as determined by SDS-PAGE.
Endotoxin	< 0.1EU per μg of the protein as determined by the LAL method.
Activity	Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC). The expected ED₅₀ for this effect is ≤20 ng/mL.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 6% mannitol are added as protectants before lyophilization.
Stability	24 months at 2℃ to 8℃ in lyophilized state. 6 months at -20℃ under sterile conditions after reconstitution. 12 months at -80℃ under sterile conditions after reconstitution.
Storage	Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL. Avoid repeated freeze-thaw cycles.