0102030405
Recombinant Human TGF-β3 Protein
Product Overview
Recombinant Human Transforming Growth Factor Beta 3 (TGF-β3) is a multifunctional cytokine involved in the regulation of cell growth, differentiation, and immune responses. By binding to its receptors, TGF-β3 activates downstream signaling pathways that regulate extracellular matrix production and tissue repair, making it an essential tool in cellular biology research.
Key Features
1.High Purity: This product is subjected to rigorous purification processes, achieving a purity level of over 95%, ensuring reliable and reproducible experimental results.
2.High Activity: Multiple activity assays confirm that this product effectively induces expected responses in target cells, suitable for various cell biology studies.
3.Batch Consistency: Strict production and quality control procedures ensure consistency between batches, reducing experimental variability.
4.Animal-Free: This product is manufactured without the use of any animal-derived components, meeting animal-free requirements and suitable for a wider range of research applications.
Applications
• Cell Biology: Used to study fundamental biological processes such as cell growth, differentiation, and apoptosis.
• Tissue Engineering: Promotes extracellular matrix formation and tissue regeneration, important in tissue repair and regenerative medicine.
• Immunology: Investigates the role of TGF-β3 in immune regulation, exploring its potential applications in the treatment of immune diseases.
• Oncology: Studies the role of TGF-β3 in the tumor microenvironment, providing new insights and strategies for cancer therapy.
Specifications and Packaging
• Specifications: Available in various sizes to meet different experimental needs, including 10μg, 50μg, 100μg, and 1mg.
• Packaging: Supplied in sterile lyophilized form for ease of storage and transport.
Storage and Stability
• Storage: Store at -20°C or below, avoiding repeated freeze-thaw cycles.
• Stability: The product is stable under recommended storage conditions, ensuring long-term usability.
Instructions for Use
1.Before use, dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment.
2.Dilute and prepare according to experimental requirements.
3.For accurate results, do not mix with other similar products.
Cautions
• This product is for research use only and is not intended for clinical diagnosis or treatment.
• Follow the instructions carefully to avoid affecting product activity.
• If you encounter any issues during use, please contact our technical support team promptly.
| Cytokines | |
| Expression Host | HEK293 cells |
| Protein Sequence | A DNA sequence encoding the human TGF-β3（P10600 A301-S412）was expressed with a Fc-tag at the C-terminus. |
| Molecular Mass | The recombinant human TGF-β3 protein consists of 375 amino acids and predicts a molecular mass of 42.1 kD. |
| QC Testing Purity | > 90 % as determined by SDS-PAGE |
| Endotoxin | < 0.1EU per 1 μg of the protein by the LAL method. |
| Activity | Determined by the dose-dependent stimulation of the inhibitory proliferation of IL-4 - dependent mouse HT-2 cells.The expected ED₅₀ for this effect is ≤ 0.1 ng/mL. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization. |
| Stability | 24 months at 2℃ to 8℃ in lyophilized state. 6 months at -20℃ under sterile conditions after reconstitution. 12 months at -80℃ under sterile conditions after reconstitution. |
| Storage | Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL. Avoid repeated freeze-thaw cycles. |
| Thumb | File information |
 |
GMP-TL646_SDS.pdf |
|
GMP-TL646_Product Sheet.pdf |