Recombinant Human LIF Protein

Stock: In Stock
Model: GMP-TL633

Description

Characterization

Document Files

Product Overview

Recombinant Human Leukemia Inhibitory Factor (LIF) is a multifunctional cytokine that plays a vital role in the regulation of cell differentiation, proliferation, and survival. LIF is involved in various physiological processes, including hematopoiesis, inflammation, and embryonic development. This protein is essential for research in stem cell biology, immunology, and oncology.

Key Features

1.High Purity: Purified using advanced chromatographic techniques, this product achieves a purity level of over 95%, ensuring accurate and reproducible experimental results.

2.High Biological Activity: Validated through multiple activity assays, this LIF protein effectively promotes cell differentiation and survival, making it suitable for a range of research applications.

3.Batch Consistency: Produced under stringent quality control measures to ensure high consistency between batches, reducing variability and enhancing experimental reliability.

4.Animal-Free: Manufactured without the use of animal-derived components, adhering to animal-free standards and broadening its applicability for diverse research needs.

Applications

• Stem Cell Research: Study the role of LIF in maintaining the pluripotency of embryonic stem cells and promoting their differentiation into various cell lineages, advancing research in regenerative medicine.

• Immunology: Investigate the effects of LIF on immune cell function and differentiation, providing insights into immune responses and potential therapeutic applications.

• Oncology: Explore the role of LIF in cancer cell biology, including its impact on tumor growth and metastasis, contributing to the development of novel cancer therapies.

• Hematopoiesis: Utilize LIF to study its role in the regulation of hematopoietic stem cells and progenitor cells, offering valuable information for the treatment of hematological disorders.

Specifications and Packaging

• Specifications: Available in various sizes to meet different experimental requirements, including 10μg, 50μg, 100μg, and 1mg.

• Packaging: Supplied in sterile lyophilized form to ensure stability and facilitate convenient storage and transportation.

Storage and Stability

• Storage: Store at -20°C or below to maintain product stability and biological activity. Avoid repeated freeze-thaw cycles to prevent degradation.

• Stability: Stable for long-term storage under recommended conditions, ensuring reliable performance in research applications.

Instructions for Use

1.Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.

2.Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.

3.Avoid mixing with other similar products to prevent cross-reactivity and potential interference.

Cautions

• This product is intended for research use only and is not suitable for clinical diagnostics or therapeutic applications.

• Handle the product according to provided instructions to preserve protein activity and integrity.

• For any issues or inquiries regarding the product, please contact our technical support team.

Cytokines
Expression Host	HEK293 cells
Synonym	CDF,DIA,HILDA,Leukemia Inhibitory Factor,MLPLI
Protein Sequence	DNA sequence encoding human LIF(NP_002300.1) expressed with His tag at the C-terminus.
Molecular Mass	The recombinant human LIF protein contains 186 amino acids and has a predicted molecular weight of 20.5kD.
QC Testing Purity	> 90 % as determined by SDS-PAGE.
Endotoxin	≤0.01EU/ug (gel method).
Activity	Determined by the expansion level of TF-1 cells. The expected ED₅₀ for this effect is ≤ 0.1ng/mL, corresponding to a specific activity of ≥1×10⁷U/mg.
Formulation
Stability	24 months at 4℃ in lyophilized state. 6 months-12 months at -20℃ under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
Storage	24 months at 4℃ in lyophilized state. 6 months-12 months at -20℃ under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.